Key features and details
- Rabbit polyclonal to SLC38A1/NAT2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SLC38A1/NAT2 antibody
See all SLC38A1/NAT2 primary antibodies
DescriptionRabbit polyclonal to SLC38A1/NAT2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Rat, Horse, Guinea pig, Cow, Cat, Dog
- HepG2 cell lysate; human kidney tissue
This product was previously labelled as SLC38A1
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab60145 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1.25 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 59 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
FunctionFunctions as a sodium-dependent amino acid transporter. Mediates the saturable, pH-sensitive and electrogenic cotransport of glutamine and sodium ions with a stoichiometry of 1:1. May also transport small zwitterionic and aliphatic amino acids with a lower affinity. May supply glutamatergic and GABAergic neurons with glutamine which is required for the synthesis of the neurotransmitters glutamate and GABA.
Tissue specificityExpressed in the cerebral cortex by pyramidal and GABAergic neurons, astrocytes and other non-neuronal cells (at protein level). Expressed in placenta, heart, lung, skeletal muscle, spleen, stomach and testis.
Sequence similaritiesBelongs to the amino acid/polyamine transporter 2 family.
Cellular localizationCell membrane. Restricted to the somatodendritic compartment of neurons. Found in the cellular processes of neurons in the developing brain (By similarity).
- Information by UniProt
- Amino acid transporter A1 antibody
- Amino acid transporter system A1 antibody
- ATA 1 antibody
Anti-SLC38A1/NAT2 antibody (ab60145) at 1.25 µg/ml + HepG2 cell lysate at 10 µg
HRP-conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 59 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SLC38A1/NAT2 with ab60145 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.
ab60145 has been referenced in 2 publications.
- Dikalova AE et al. Reactive oxygen species modulate Na+-coupled neutral amino acid transporter 1 expression in piglet pulmonary arterial endothelial cells. Am J Physiol Heart Circ Physiol 316:H911-H919 (2019). PubMed: 30794434
- Dikalova A et al. Sodium-coupled neutral amino acid transporter 1 (SNAT1) modulates L-citrulline transport and nitric oxide (NO) signaling in piglet pulmonary arterial endothelial cells. PLoS One 9:e85730 (2014). WB ; Pig . PubMed: 24454923