Overview

  • Product name

    Anti-SLC38A1/NAT2 antibody [N104/32]
    See all SLC38A1/NAT2 primary antibodies
  • Description

    Mouse monoclonal [N104/32] to SLC38A1/NAT2
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein corresponding to Rat SLC38A1/NAT2 aa 1-63 (N terminal).
    Sequence:

    MMHFKSGLEL TELQNMTVPE DDNVSNDSND FTEVENGQIN SKFISDRESR RSLTNSHLEK RKC


    Database link: NP_620187.1

  • Positive control

    • WB: Rat brain membrane tissue lysate; lysate from neocortical neurons cultured under amino acid starvation conditions. ICC/IF: SK-N-BE cells.
  • General notes

    The clone number has been updated from S104-32 to N104/32, both clone numbers name the same antibody clone.

    This product was previously labelled as SLC38A1.

Properties

Applications

Our Abpromise guarantee covers the use of ab134268 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 54 kDa.

1µg/ml of ab134268 is sufficient for detection of SLC38A1/NAT2 in 20µg of lysates from neocortical neurons cultured under amino acid starvation conditions.

IHC-P Use at an assay dependent concentration.
ICC/IF 1/100.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function

    Functions as a sodium-dependent amino acid transporter. Mediates the saturable, pH-sensitive and electrogenic cotransport of glutamine and sodium ions with a stoichiometry of 1:1. May also transport small zwitterionic and aliphatic amino acids with a lower affinity. May supply glutamatergic and GABAergic neurons with glutamine which is required for the synthesis of the neurotransmitters glutamate and GABA.
  • Tissue specificity

    Expressed in the cerebral cortex by pyramidal and GABAergic neurons, astrocytes and other non-neuronal cells (at protein level). Expressed in placenta, heart, lung, skeletal muscle, spleen, stomach and testis.
  • Sequence similarities

    Belongs to the amino acid/polyamine transporter 2 family.
  • Cellular localization

    Cell membrane. Restricted to the somatodendritic compartment of neurons. Found in the cellular processes of neurons in the developing brain (By similarity).
  • Information by UniProt
  • Database links

  • Alternative names

    • Amino acid transporter A1 antibody
    • Amino acid transporter system A1 antibody
    • ATA 1 antibody
    • ATA1 antibody
    • N-system amino acid transporter 2 antibody
    • NAT 2 antibody
    • NAT2 antibody
    • S38A1_HUMAN antibody
    • SAT 1 antibody
    • SAT1 antibody
    • SLC38A1 antibody
    • SNAT 1 antibody
    • SNAT1 antibody
    • Sodium-coupled neutral amino acid transporter 1 antibody
    • Solute carrier family 38 member 1 antibody
    • System A amino acid transporter 1 antibody
    • System N amino acid transporter 1 antibody
    see all

Images

  • 4% formaldehyde-fixed SK-N-BE cells stained for SLC38A1/NAT2 (green) using ab134268 at 1/100 dilution in ICC/IF.

    Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/100 for 60 min at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000; 1/5000 for 60 min room temperature, 5 min room temperature.

    A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ab134268. (D) Composite.

  • Anti-SLC38A1/NAT2 antibody [N104/32] (ab134268) at 1/1000 dilution + Rat brain membrane tissue lysate

    Secondary
    Goat anti-mouse IgG HRP

    Predicted band size: 54 kDa

References

This product has been referenced in:

  • Kitada K  et al. High salt intake reprioritizes osmolyte and energy metabolism for body fluid conservation. J Clin Invest 127:1944-1959 (2017). Read more (PubMed: 28414295) »
See 1 Publication for this product

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