Key features and details
- Mouse monoclonal [N104/32] to SLC38A1/NAT2
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Product nameAnti-SLC38A1/NAT2 antibody [N104/32]
See all SLC38A1/NAT2 primary antibodies
DescriptionMouse monoclonal [N104/32] to SLC38A1/NAT2
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
- WB: Rat brain membrane tissue lysate; lysate from neocortical neurons cultured under amino acid starvation conditions. ICC/IF: SK-N-BE cells.
The clone number has been updated from S104-32 to N104/32, both clone numbers name the same antibody clone.
This product was previously labelled as SLC38A1.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab134268 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 54 kDa.
1µg/ml of ab134268 is sufficient for detection of SLC38A1/NAT2 in 20µg of lysates from neocortical neurons cultured under amino acid starvation conditions.
FunctionFunctions as a sodium-dependent amino acid transporter. Mediates the saturable, pH-sensitive and electrogenic cotransport of glutamine and sodium ions with a stoichiometry of 1:1. May also transport small zwitterionic and aliphatic amino acids with a lower affinity. May supply glutamatergic and GABAergic neurons with glutamine which is required for the synthesis of the neurotransmitters glutamate and GABA.
Tissue specificityExpressed in the cerebral cortex by pyramidal and GABAergic neurons, astrocytes and other non-neuronal cells (at protein level). Expressed in placenta, heart, lung, skeletal muscle, spleen, stomach and testis.
Sequence similaritiesBelongs to the amino acid/polyamine transporter 2 family.
Cellular localizationCell membrane. Restricted to the somatodendritic compartment of neurons. Found in the cellular processes of neurons in the developing brain (By similarity).
- Information by UniProt
- Amino acid transporter A1 antibody
- Amino acid transporter system A1 antibody
- ATA 1 antibody
4% formaldehyde-fixed SK-N-BE cells stained for SLC38A1/NAT2 (green) using ab134268 at 1/100 dilution in ICC/IF.
Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/100 for 60 min at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000; 1/5000 for 60 min room temperature, 5 min room temperature.
A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ab134268. (D) Composite.
Anti-SLC38A1/NAT2 antibody [N104/32] (ab134268) at 1/1000 dilution + Rat brain membrane tissue lysate
Goat anti-mouse IgG HRP
Predicted band size: 54 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab134268 has been referenced in 1 publication.
- Kitada K et al. High salt intake reprioritizes osmolyte and energy metabolism for body fluid conservation. J Clin Invest 127:1944-1959 (2017). PubMed: 28414295