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    slc38a3sn1-antibody-ab211516.pdf

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Signal Transduction Metabolism Amino Acids
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Anti-SLC38A3/SN1 antibody (ab211516)

  • Datasheet
Reviews (1) Submit a question References (1)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC38A3/SN1 antibody (ab211516)
  • Western blot - Anti-SLC38A3/SN1 antibody (ab211516)
  • Flow Cytometry - Anti-SLC38A3/SN1 antibody (ab211516)

Key features and details

  • Rabbit polyclonal to SLC38A3/SN1
  • Suitable for: WB, Flow Cyt, IHC-P
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-SLC38A3/SN1 antibody
    See all SLC38A3/SN1 primary antibodies
  • Description

    Rabbit polyclonal to SLC38A3/SN1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SLC38A3/SN1 aa 442-468 (internal sequence) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: Q99624

  • Positive control

    • Human kidney tissue. HepG2 cells and cell line lysates.
  • General notes

     This product was previously labelled as SLC38A3

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Ammonium Sulphate Precipitation
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HepG2 whole cell lysate (ab166833)

Applications

Our Abpromise guarantee covers the use of ab211516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 56 kDa.
Flow Cyt 1/10 - 1/50.
IHC-P 1/50.

Target

  • Relevance

    SLC38A3 is a sodium dependent amino acid/proton antiporter. It mediates electrogenic cotransport of glutamine and sodium ions in exchange for protons. It also recognizes histidine, asparagine and alanine. SLC38A3 may mediate amino acid transport in either direction under physiological conditions an may play a role in nitrogen metabolism and synaptic transmission.
  • Cellular localization

    Cell membrane; Multipass membrane protein
  • Database links

    • Entrez Gene: 10991 Human
    • Omim: 604437 Human
    • SwissProt: Q99624 Human
    • Unigene: 76460 Human
    • Alternative names

      • G17 antibody
      • N system amino acid transporter 1 antibody
      • N-system amino acid transporter 1 antibody
      • Na(+)-coupled neutral amino acid transporter 3 antibody
      • NAT1 antibody
      • SN1 antibody
      • SNAT3 antibody
      • Sodium coupled neutral amino acid transporter 3 antibody
      • Sodium-coupled neutral amino acid transporter 3 antibody
      • Solute carrier family 38 member 3 antibody
      • System N amino acid transporter 1 antibody
      • System N1 Na+ and H+ coupled glutamine transporter antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC38A3/SN1 antibody (ab211516)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC38A3/SN1 antibody (ab211516)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling SLC38A3/SN1 with ab211516 at 1/50 dilution.

    • Western blot - Anti-SLC38A3/SN1 antibody (ab211516)
      Western blot - Anti-SLC38A3/SN1 antibody (ab211516)
      Anti-SLC38A3/SN1 antibody (ab211516) at 1/1000 dilution + HepG2 cell line lysates at 35 µg

      Predicted band size: 56 kDa

    • Flow Cytometry - Anti-SLC38A3/SN1 antibody (ab211516)
      Flow Cytometry - Anti-SLC38A3/SN1 antibody (ab211516)

      Flow cytometric analysis of HepG2 cells labeling SLC38A3/SN1 with ab211516 at 1/10 dilution (lower histogram) compared to a negative control cell (upper histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab211516? Please let us know so that we can cite the reference in this datasheet.

    ab211516 has been referenced in 1 publication.

    • Baek JH  et al. Glutamine Supplementation Ameliorates Chronic Stress-induced Reductions in Glutamate and Glutamine Transporters in the Mouse Prefrontal Cortex. Exp Neurobiol 28:270-278 (2019). PubMed: 31138994

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-SLC38A3 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (prefrontal cortex)
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Loading amount
    10 µg
    Specification
    prefrontal cortex
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 19 2017

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