Anti-SLC38A3/SN1 antibody (ab211516)
Key features and details
- Rabbit polyclonal to SLC38A3/SN1
- Suitable for: WB, Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-SLC38A3/SN1 antibody
See all SLC38A3/SN1 primary antibodies -
Description
Rabbit polyclonal to SLC38A3/SN1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human SLC38A3/SN1 aa 442-468 (internal sequence) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q99624 -
Positive control
- Human kidney tissue. HepG2 cells and cell line lysates.
-
General notes
This product was previously labelled as SLC38A3
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab211516 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 56 kDa. | |
| Flow Cyt | 1/10 - 1/50. | |
| IHC-P | 1/50. |
Target
-
Relevance
SLC38A3 is a sodium dependent amino acid/proton antiporter. It mediates electrogenic cotransport of glutamine and sodium ions in exchange for protons. It also recognizes histidine, asparagine and alanine. SLC38A3 may mediate amino acid transport in either direction under physiological conditions an may play a role in nitrogen metabolism and synaptic transmission. -
Cellular localization
Cell membrane; Multipass membrane protein -
Database links
- Entrez Gene: 10991 Human
- Omim: 604437 Human
- SwissProt: Q99624 Human
- Unigene: 76460 Human
-
Alternative names
- G17 antibody
- N system amino acid transporter 1 antibody
- N-system amino acid transporter 1 antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC38A3/SN1 antibody (ab211516)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling SLC38A3/SN1 with ab211516 at 1/50 dilution.
-
Western blot - Anti-SLC38A3/SN1 antibody (ab211516)Anti-SLC38A3/SN1 antibody (ab211516) at 1/1000 dilution + HepG2 cell line lysates at 35 µg
Predicted band size: 56 kDa -
Flow Cytometry - Anti-SLC38A3/SN1 antibody (ab211516)Flow cytometric analysis of HepG2 cells labeling SLC38A3/SN1 with ab211516 at 1/10 dilution (lower histogram) compared to a negative control cell (upper histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protocols
Datasheets and documents
References (1)
ab211516 has been referenced in 1 publication.
- Baek JH et al. Glutamine Supplementation Ameliorates Chronic Stress-induced Reductions in Glutamate and Glutamine Transporters in the Mouse Prefrontal Cortex. Exp Neurobiol 28:270-278 (2019). PubMed: 31138994
