Overview

  • Product name

    Anti-SLC38A4 antibody
  • Description

    Rabbit polyclonal to SLC38A4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Caenorhabditis elegans, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide within Human SLC38A4 aa 381-430. The exact sequence is proprietary.

  • Positive control

    • Jurkat cell lysate; human kidney tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab58785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Predicted molecular weight: 61 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • Relevance

    SLC38A4 is found predominantly in liver and transports both cationic and neutral amino acids. The transport of cationic amino acids by SLC38A4 is Na+ and pH independent, while the transport of neutral amino acids is Na+ and pH dependent. It has a broad specificity, with a preference for Ala, followed by His, Cys, Asn, Ser, Gly, Val, Thr, Gln and Met.
  • Cellular localization

    Cell Membrane; Multi-pass membrane protein
  • Database links

  • Alternative names

    • Amino acid transporter A3 antibody
    • Na(+)-coupled neutral amino acid transporter 4 antibody
    • Sodium-coupled neutral amino acid transporter 4 antibody
    • Solute carrier family 38 member 4 antibody
    • System N amino acid transporter 3 antibody
    see all

Images

  • Lane 1 : marker
    Lane 2 : Anti-SLC38A4 antibody (ab58785) at 2.5 µg/ml

    Lane 2 : Jurkat cell lysate at 10 µg with 5% skim milk in PBS buffer

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 61 kDa



    Gel concentration 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SLC38A4 with ab58785 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of renal tubule. Magnification: 400X.

References

This product has been referenced in:

  • Shang M & Wen Z Increased placental IGF-1/mTOR activity in macrosomia born to women with gestational diabetes. Diabetes Res Clin Pract 146:211-219 (2018). Read more (PubMed: 30389621) »
  • Desforges M  et al. The SNAT4 isoform of the system A amino acid transporter is functional in human placental microvillous plasma membrane. J Physiol 587:61-72 (2009). WB, IHC-P ; Human . Read more (PubMed: 19015196) »
See all 2 Publications for this product

Customer reviews and Q&As

Answer



Unfortunately, at this time, the only data our lab has is the western blot and IHC data with human samples. We have not conducted additional experiments to address why the staining seems to be mainly cytoplasmic. Additionally, from the 14aa peptide used to raise this antibody, the only human protein that it is 100% homologous to is the SLC38A4 (http://www.uniprot.org/uniprot/Q969I6).

Due to a high sequence homology of 86% between the immunogen used and the mouse protein, we have predicted that this antibody will work in mouse however, we have not tested this and therefore, cannot guarantee this. If you would like to test this product in mouse, we could offer you a testing discount.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up