Anti-SLC38A5 / SNAT5 antibody (ab72717)
Key features and details
- Rabbit polyclonal to SLC38A5 / SNAT5
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-SLC38A5 / SNAT5 antibody -
Description
Rabbit polyclonal to SLC38A5 / SNAT5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Human
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Immunogen
Synthetic peptide corresponding to Rat SLC38A5/ SNAT5 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab92656) -
Positive control
- This antibody gave a positive signal in the following rat tissue lysates: Cortex; Hippocampus; Spinal Cord. This antibody gave a positive result in IHC in the following FFPE tissue: Rat normal pancreas.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72717 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
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| IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use at an assay dependent concentration.
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| Notes |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Functions as a sodium-dependent amino acid transporter which countertransport protons. Mediates the saturable, pH-sensitive, and electrogenic cotransport of several neutral amino acids including glycine, asparagine, alanine, serine, glutamine and histidine with sodium. -
Tissue specificity
Predominantly expressed in stomach, brain, liver, lung and intestinal tract. -
Sequence similarities
Belongs to the amino acid/polyamine transporter 2 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 92745 Human
- Entrez Gene: 209837 Mouse
- Entrez Gene: 192208 Rat
- Omim: 300649 Human
- SwissProt: Q8WUX1 Human
- SwissProt: Q3U1J0 Mouse
- SwissProt: A2VCW5 Rat
- Unigene: 195155 Human
see all -
Alternative names
- Amino acid transporter system N2 antibody
- JM 24 antibody
- JM24 antibody
see all
Images
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Western blot - Anti-SLC38A5 / SNAT5 antibody (ab72717)All lanes : Anti-SLC38A5 / SNAT5 antibody (ab72717) at 1 µg/ml
Lane 1 : Cortex (Rat) Tissue Lysate
Lane 2 : Hippocampus (Rat) Tissue Lysate
Lane 3 : Spinal Cord (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 52 kDa
Additional bands at: 28 kDa, 30 kDa, 40 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC38A5 / SNAT5 antibody (ab72717)IHC image of SLC38A5 / SNAT5 staining in Rat normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72717, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunocytochemistry/ Immunofluorescence - Anti-SLC38A5 / SNAT5 antibody (ab72717)ICC/IF image of ab72717 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72717, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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Datasheet download
References (7)
ab72717 has been referenced in 7 publications.
- Smith DK et al. Elevated Serum Amino Acids Induce a Subpopulation of Alpha Cells to Initiate Pancreatic Neuroendocrine Tumor Formation. Cell Rep Med 1:100058 (2020). PubMed: 33205067
- McIntyre KR et al. Human placental uptake of glutamine and glutamate is reduced in fetal growth restriction. Sci Rep 10:16197 (2020). PubMed: 33004923
- Liao M et al. LncRNA EPB41L4A-AS1 regulates glycolysis and glutaminolysis by mediating nucleolar translocation of HDAC2. EBioMedicine 41:200-213 (2019). PubMed: 30796006
- Baek JH et al. Glutamine Supplementation Ameliorates Chronic Stress-induced Reductions in Glutamate and Glutamine Transporters in the Mouse Prefrontal Cortex. Exp Neurobiol 28:270-278 (2019). PubMed: 31138994
- McIntyre KR et al. Evidence of adaptation of maternofetal transport of glutamine relative to placental size in normal mice, and in those with fetal growth restriction. J Physiol 597:4975-4990 (2019). PubMed: 31400764
- Kim J et al. Amino Acid Transporter Slc38a5 Controls Glucagon Receptor Inhibition-Induced Pancreatic a Cell Hyperplasia in Mice. Cell Metab 25:1348-1361.e8 (2017). PubMed: 28591637
- Day PE et al. Partitioning of glutamine synthesised by the isolated perfused human placenta between the maternal and fetal circulations. Placenta 34:1223-31 (2013). WB ; Human . PubMed: 24183194
