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  1. Link

    slc38a9-antibody-ab130398.pdf

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Signal Transduction Metabolism Amino Acids
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Anti-SLC38A9 antibody (ab130398)

  • Datasheet
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  • Western blot - Anti-SLC38A9 antibody (ab130398)
  • Immunocytochemistry/ Immunofluorescence - Anti-SLC38A9 antibody (ab130398)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC38A9 antibody (ab130398)

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  • Datasheet
  • References
  • Protocols

Overview

  • Product name

    Anti-SLC38A9 antibody
    See all SLC38A9 primary antibodies
  • Description

    Rabbit polyclonal to SLC38A9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 5-105 of Human SLC38A9 (UniProt Q8NBW4).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human Plasma and Human liver tissue
  • General notes

    The antibody solution should be gently mixed before use.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • Channels
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

Our Abpromise guarantee covers the use of ab130398 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/250 - 1/500. Predicted molecular weight: 64 kDa.
IHC-P 1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Putative sodium-dependent amino acid/proton antiporter.
  • Sequence similarities

    Belongs to the amino acid/polyamine transporter 2 family.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession Q8NBW4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 153129 Human
    • SwissProt: Q8NBW4 Human
    • Unigene: 726519 Human
    • Alternative names

      • FLJ46104 antibody
      • FLJ90709 antibody
      • Homo sapiens solute carrier family 38, member 9 antibody
      • MGC120544 antibody
      • OTTHUMP00000161835 antibody
      • Putative sodium coupled neutral amino acid transporter 9 antibody
      • Putative sodium-coupled neutral amino acid transporter 9 antibody
      • Putative uncharacterized protein FLJ90709 antibody
      • S38A9_HUMAN antibody
      • SLC38A9 antibody
      see all

    Images

    • Western blot - Anti-SLC38A9 antibody (ab130398)
      Western blot - Anti-SLC38A9 antibody (ab130398)
      All lanes : Anti-SLC38A9 antibody (ab130398) at 1/250 dilution

      Lane 1 : RT4 cell lysate
      Lane 2 : U-251 MG cell lysate
      Lane 3 : Human Plasma lysate
      Lane 4 : Human Liver lysate
      Lane 5 : Human Tonsil lysate

      Predicted band size: 64 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-SLC38A9 antibody (ab130398)
      Immunocytochemistry/ Immunofluorescence - Anti-SLC38A9 antibody (ab130398)
      Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus but not nucleoli and vesicles. Recommended concentration of ab130398 1-4 µg/ml. Cells treated with PFA/Triton X-100.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC38A9 antibody (ab130398)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC38A9 antibody (ab130398)
      ab130398, at a dilution of 1/1000, staining SLC38A9 in paraffin-embedded Human liver tissue by Immunohistochemistry.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Datasheets and documents

    • Datasheet
  • References

    ab130398 has not yet been referenced specifically in any publications.

    Publishing research using ab130398? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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