Anti-SLC39A14/ZIP-14 antibody (ab106568)
Key features and details
- Rabbit polyclonal to SLC39A14/ZIP-14
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SLC39A14/ZIP-14 antibody
See all SLC39A14/ZIP-14 primary antibodies -
Description
Rabbit polyclonal to SLC39A14/ZIP-14 -
Host species
Rabbit -
Specificity
At least three isoforms of SLC39A14/ZIP-14 are known to exist; this antibody will detect all isoforms.
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Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human spleen tissue lysate. IHC-P: Mouse liver tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106568 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 54 kDa.
WB suitable for Human samples only |
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IHC-P |
Use a concentration of 2.5 - 20 µg/ml.
IHC-P suitable for Mouse samples only |
Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 54 kDa. WB suitable for Human samples only |
IHC-P
Use a concentration of 2.5 - 20 µg/ml. IHC-P suitable for Mouse samples only |
Target
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Function
May be able to transport iron (By similarity). Acts as a zinc-influx transporter. -
Tissue specificity
Ubiquitously expressed, with increased expression in liver, pancreas, fetal liver, thyroid gland, left and right ventricle, right atrium and fetal heart. Weakly expressed in spleen, thymus, and peripheral blood leukocytes. -
Sequence similarities
Belongs to the ZIP transporter (TC 2.A.5) family. -
Cellular localization
Cell membrane. Cell projection > lamellipodium. Localized to the plasma membrane and also found colocalized with F-actin concentrated on lamellipodiae. - Information by UniProt
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Database links
- Entrez Gene: 23516 Human
- Entrez Gene: 213053 Mouse
- Omim: 608736 Human
- SwissProt: Q15043 Human
- SwissProt: Q75N73 Mouse
- Unigene: 491232 Human
- Unigene: 270647 Mouse
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Alternative names
- Cig19 antibody
- KIAA0062 antibody
- LIV-1 subfamily of ZIP zinc transporter 4 antibody
see all
Images
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All lanes : Anti-SLC39A14/ZIP-14 antibody (ab106568)
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : SLC39A14 knockout HEK-293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-SLC39A14/ZIP-14 antibody staining at 1 μg/ml, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab106568 was shown to bind specifically to SLC39A14/ZIP-14. A band was observed at 68 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in SLC39A14 knockout cell line ab266126 (knockout cell lysate ab258683). To generate this image, wild-type and SLC39A14 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween®20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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Lane 1 : Anti-SLC39A14/ZIP-14 antibody (ab106568) at 1 µg/ml
Lane 2 : Anti-SLC39A14/ZIP-14 antibody (ab106568) at 2 µg/ml
All lanes : Human spleen tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 54 kDa -
Immunohistochemical analysis of SLC39A14/ZIP-14 in mouse liver tissue using ab106568 at 20 µg/ml.
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Immunohistochemistry of mouse liver tissue using ab106568 at 2.5 μg/ml
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab106568 has been referenced in 5 publications.
- Chen Z et al. Zinc ameliorates human aortic valve calcification through GPR39 mediated ERK1/2 signalling pathway. Cardiovasc Res 117:820-835 (2021). PubMed: 32259211
- Routhe LJ et al. Astrocytic expression of ZIP14 (SLC39A14) is part of the inflammatory reaction in chronic neurodegeneration with iron overload. Glia 68:1810-1823 (2020). PubMed: 32077535
- Liu X et al. Lysosomal dysfunction is associated with persistent lung injury in dams caused by pregnancy exposure to carbon black nanoparticles. Life Sci 233:116741 (2019). PubMed: 31398419
- Wongjaikam S et al. Restoring the impaired cardiac calcium homeostasis and cardiac function in iron overload rats by the combined deferiprone and N-acetyl cysteine. Sci Rep 7:44460 (2017). PubMed: 28287621
- Tuschl K et al. Mutations in SLC39A14 disrupt manganese homeostasis and cause childhood-onset parkinsonism-dystonia. Nat Commun 7:11601 (2016). IHC . PubMed: 27231142