Recombinant
RabMAb

Recombinant Anti-SLC39A6/ZIP-6 antibody [SP300] (ab227696)

Overview

  • Product name

    Anti-SLC39A6/ZIP-6 antibody [SP300]
    See all SLC39A6/ZIP-6 primary antibodies
  • Description

    Rabbit monoclonal [SP300] to SLC39A6/ZIP-6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human SLC39A6/ZIP-6 aa 200-300. The exact sequence is proprietary.
    Database link: Q13433

  • Positive control

    • IHC-P: Human breast ductal carcinoma, uterus, and Human breast carcinoma tissue; WB: ZR-75-1 cell lysate; FC: PANC-1 cells.
  • General notes

    Previously labelled as SLC39A6. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab227696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.

Perform heat mediated antigen retrieval by boiling tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Primary antibody incubation for 10 minutes at room temperature.

WB 1/400. Predicted molecular weight: 85 kDa.

Primary antibody incubation for 1 hour at room temperature.

Flow Cyt 1/400.

Primary antibody incubation for 30 minutes at at 4°C.

Target

  • Function

    May act as a zinc-influx transporter.
  • Tissue specificity

    Highly expressed in the breast, prostate, placenta, kidney, pituitary and corpus callosum. Weakly expressed in heart and intestine. Also highly expressed in cells derived from an adenocarcinoma of the cervix and lung carcinoma.
  • Sequence similarities

    Belongs to the ZIP transporter (TC 2.A.5) family.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Estrogen regulated protein LIV 1 6 antibody
    • Estrogen-regulated protein LIV-1 antibody
    • LIV 1 antibody
    • LIV 1 protein, estrogen regulated antibody
    • S39A6_HUMAN antibody
    • SLC39A6 antibody
    • Solute carrier family 39 (metal ion transporter), member 6 antibody
    • solute carrier family 39 (zinc transporter), member 6 antibody
    • Solute carrier family 39 member 6 antibody
    • Zinc transporter ZIP6 antibody
    • ZIP 6 antibody
    • ZIP-6 antibody
    • Zrt and Irt like protein 6 antibody
    • Zrt- and Irt-like protein 6 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling SLC39A6/ZIP-6 with ab227696 at 1/100 dilution (4.05 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Formalin-fixed, paraffin-embedded human uterus tissue stained for SLC39A6/ZIP-6 using ab227696 at 1/100 dilution in immunohistochemical analysis.

  • Anti-SLC39A6/ZIP-6 antibody [SP300] (ab227696) at 1/400 dilution + ZR-75-1 cell lysate

    Predicted band size: 85 kDa

  • Flow cytometric analysis of PANC-1 (human pancreatic epithelial carcinoma cell line) cell line labeling SLC39A6/ZIP-6 with ab227696 at 1/400 dilution (green) compared with a negative control of rabbit IgG (blue).

  • Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for SLC39A6/ZIP-6 using ab227696 at 1/100 dilution in immunohistochemical analysis.

References

ab227696 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227696.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up