Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP300] to SLC39A6/ZIP-6
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Human
Product nameAnti-SLC39A6/ZIP-6 antibody [SP300]
See all SLC39A6/ZIP-6 primary antibodies
DescriptionRabbit monoclonal [SP300] to SLC39A6/ZIP-6
Tested applicationsSuitable for: IHC-P, WB, Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human SLC39A6/ZIP-6 aa 200-300. The exact sequence is proprietary.
Database link: Q13433
- IHC-P: Human breast ductal carcinoma, uterus, and Human breast carcinoma tissue; WB: ZR-75-1 cell lysate; Flow Cyt: PANC-1 cells.
FOR RESEARCH USE ONLY. For commercial use, please contact email@example.com.
Previously labelled as SLC39A6.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab227696 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Perform heat mediated antigen retrieval by boiling tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.
Primary antibody incubation for 10 minutes at room temperature.
|WB||1/400. Predicted molecular weight: 85 kDa.
Primary antibody incubation for 1 hour at room temperature.
Primary antibody incubation for 30 minutes at at 4°C.
FunctionMay act as a zinc-influx transporter.
Tissue specificityHighly expressed in the breast, prostate, placenta, kidney, pituitary and corpus callosum. Weakly expressed in heart and intestine. Also highly expressed in cells derived from an adenocarcinoma of the cervix and lung carcinoma.
Sequence similaritiesBelongs to the ZIP transporter (TC 2.A.5) family.
Cellular localizationCell membrane.
- Information by UniProt
- Estrogen regulated protein LIV 1 6 antibody
- Estrogen-regulated protein LIV-1 antibody
- LIV 1 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling SLC39A6/ZIP-6 with ab227696 at 1/100 dilution (4.05 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Formalin-fixed, paraffin-embedded human uterus tissue stained for SLC39A6/ZIP-6 using ab227696 at 1/100 dilution in immunohistochemical analysis.
Anti-SLC39A6/ZIP-6 antibody [SP300] (ab227696) at 1/400 dilution + ZR-75-1 cell lysate
Predicted band size: 85 kDa
Flow cytometric analysis of PANC-1 (human pancreatic epithelial carcinoma cell line) cell line labeling SLC39A6/ZIP-6 with ab227696 at 1/400 dilution (green) compared with a negative control of rabbit IgG (blue).
Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for SLC39A6/ZIP-6 using ab227696 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227696 has not yet been referenced specifically in any publications.