Anti-SLC40A1 antibody (ab235166)
Key features and details
- Rabbit polyclonal to SLC40A1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SLC40A1 antibody
See all SLC40A1 primary antibodies -
Description
Rabbit polyclonal to SLC40A1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: CACO2 whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab235166 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 5 µg/ml. Predicted molecular weight: 63 kDa.
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Notes |
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WB
Use a concentration of 5 µg/ml. Predicted molecular weight: 63 kDa. |
Target
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Function
May be involved in iron export from duodenal epithelial cell and also in transfer of iron between maternal and fetal circulation. Mediates iron efflux in the presence of a ferroxidase (hephaestin and/or ceruloplasmin). -
Tissue specificity
Expressed in placenta, intestine, muscle and spleen. -
Involvement in disease
Defects in SLC40A1 are the cause of hemochromatosis type 4 (HFE4) [MIM:606069]. HFE4 is an autosomal dominant iron-loading disorder characterized by early iron accumulation in reticuloendothelial cells and a marked increase in serum ferritin before elevation of the transferrin saturation. -
Sequence similarities
Belongs to the SLC40A transporter family. -
Cellular localization
Cell membrane. Localized to the basolateral membrane of polarized epithelial cells. - Information by UniProt
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Database links
- Entrez Gene: 30061 Human
- Entrez Gene: 53945 Mouse
- Entrez Gene: 170840 Rat
- Omim: 604653 Human
- SwissProt: Q9NP59 Human
- SwissProt: Q9JHI9 Mouse
- SwissProt: Q923U9 Rat
- Unigene: 643005 Human
see all -
Alternative names
- Ferroportin 1 antibody
- Ferroportin antibody
- Ferroportin-1 antibody
see all
Images
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All lanes : Anti-SLC40A1 antibody (ab235166) at 5 µg/ml
Lane 1 : CACO2 (Human colorectal adenocarcinoma cell line) whole cell lysate
Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 35 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG [H&L] HRP at 1/70000 dilution
Predicted band size: 63 kDa
Exposure time: 40 seconds
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab235166 has not yet been referenced specifically in any publications.