Key features and details
- Rabbit polyclonal to SLC40A1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SLC40A1 antibody
See all SLC40A1 primary antibodies
DescriptionRabbit polyclonal to SLC40A1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide. This information is considered to be commercially sensitive.
- WB: CACO2 whole cell lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab235166 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 5 µg/ml. Predicted molecular weight: 63 kDa.|
FunctionMay be involved in iron export from duodenal epithelial cell and also in transfer of iron between maternal and fetal circulation. Mediates iron efflux in the presence of a ferroxidase (hephaestin and/or ceruloplasmin).
Tissue specificityExpressed in placenta, intestine, muscle and spleen.
Involvement in diseaseDefects in SLC40A1 are the cause of hemochromatosis type 4 (HFE4) [MIM:606069]. HFE4 is an autosomal dominant iron-loading disorder characterized by early iron accumulation in reticuloendothelial cells and a marked increase in serum ferritin before elevation of the transferrin saturation.
Sequence similaritiesBelongs to the SLC40A transporter family.
Cellular localizationCell membrane. Localized to the basolateral membrane of polarized epithelial cells.
- Information by UniProt
- Ferroportin 1 antibody
- Ferroportin antibody
- Ferroportin-1 antibody
All lanes : Anti-SLC40A1 antibody (ab235166) at 5 µg/ml
Lane 1 : CACO2 (Human colorectal adenocarcinoma cell line) whole cell lysate
Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 35 µg per lane.
All lanes : Goat anti-Rabbit IgG [H&L] HRP at 1/70000 dilution
Predicted band size: 63 kDa
Exposure time: 40 seconds
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235166 has not yet been referenced specifically in any publications.