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I have tried this replacement antibody with the same western blot techniques as described earlier. Attached is the result. The first two lanes are brain lysate. The last lane is gut lysate. Do you have any suggestions?
Asked on Mar 27 2012
Thank you for your reply.
I am sorry that you are having issues with these antibodies in western blot. Having looked over all of the protocol information you sent previously and I cannot think of anything else that may prove useful. I was just curious what the longest exposure time that you did was and if you did expose for up to an hour, was there even a hint of a signal.
Since 2 different primary antibodies have not proved to be successful for you, then if you would like I can refund the cost of the original antibody ab85370, as I am not sure that another antibody would be more successful.
Please let me know if you would like me to proceed with the refund.
Answered on Mar 27 2012