Product nameAnti-SLC43A3 antibody
DescriptionRabbit polyclonal to SLC43A3
Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Horse, Dog
- Jurkat cells; Lung tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab60302 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.
Titre using peptide based assay: 1:1562500.
|WB||Use a concentration of 2.5 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 55 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
RelevanceSLC43A3 is a putative transporter, belonging to the SLC43A transporter family. It is highly expressed in macrophages.
Cellular localizationMulti-pass membrane protein
- DKFZp762A227 antibody
- EEG1 antibody
- FOAP 13 antibody
All lanes : Anti-SLC43A3 antibody (ab60302) at 2.5 µg/ml
Lane 1 : Molecular weight marker
Lane 2 : Jurkat cell lysate at 10 µg
All lanes : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 55 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Gel concentration: 12%
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling SLC43A3 with ab60302 at 4-8µg/ml. Arrows indicate positively labelled alveolar cells. Magnification: 400X.
ab60302 has not yet been referenced specifically in any publications.