RecombinantRabMAb

Recombinant Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)

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Western blot - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)
  • Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4795(2)] to SLC45A3
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-SLC45A3 antibody [EPR4795(2)]
    See all SLC45A3 primary antibodies

  • Description

    Rabbit monoclonal [EPR4795(2)] to SLC45A3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human SLC45A3 aa 400-500. The exact sequence is proprietary.
    Database link: Q96JT2

  • Positive control

    • WB: Human prostate tissue lysate; IHC-P: Human benign prostatic adenocarcinoma, prostatic hyperplasia and prostate carcinoma tissues.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product was previously labelled as Prostein.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab137065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 59 kDa).
IHC-P (1)
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurifed use at 1/100 - 1/250
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 59 kDa).
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurifed use at 1/100 - 1/250

Target

  • Tissue specificity

    Prostate specific. Expressed in all prostatic glandular cells. Expressed both in normal and cancerous prostates.

  • Sequence similarities

    Belongs to the glycoside-pentoside-hexuronide (GPH) cation symporter transporter (TC 2.A.2) family.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • IPCA 2 antibody
      • IPCA 8 antibody
      • IPCA6 antibody
      • PCANAP2 antibody
      • PCANAP6 antibody
      • PCANAP8 antibody
      • Prostate cancer associated gene 2 antibody
      • Prostate cancer associated gene 6 antibody
      • Prostate cancer associated gene 8 antibody
      • Prostate cancer associated protein 2 antibody
      • Prostate cancer associated protein 6 antibody
      • Prostate cancer associated protein 8 antibody
      • Prostate cancer-associated protein 6 antibody
      • Prostein antibody
      • PRST antibody
      • S45A3_HUMAN antibody
      • Slc45a3 antibody
      • Solute carrier family 45 member 3 antibody
      see all

    Images

    • Western blot - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)
      Western blot - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)
      Anti-SLC45A3 antibody [EPR4795(2)] (ab137065) at 1/1000 dilution (Purified) + Human prostate lysate at 15 µg

      Secondary
      Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 59 kDa
      Observed band size: 55 kDa why is the actual band size different from the predicted?



      Blocking/Diluting buffer: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue sections labeling SLC45A3 with purified ab137065 at 1/1000 dilution (0.128 µg/mL). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)

      Immunohistochemical analysis of paraffin-embedded Human prostatic adenocarcinoma tissue labelling SLC45A3 with as137065, unpurified, at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)This image is courtesy of an abreview submitted by Faye Doherty

      Immunohistochemical analysis of human prostate tissue sections labelling SLC45A3 with ab137065, unpurified, at a dilution of 1/2000. The sections were fixed with 10% NBF. The secondary antibody used was HRP conjugated goat anti rabbit IgG. Antigen retrieval was heat mediated using Tris/EDTA pH 9.0.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)

      Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labelling SLC45A3 with ab137065, unpurified, at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)
      Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)

    References (1)

    Publishing research using ab137065? Please let us know so that we can cite the reference in this datasheet.

    ab137065 has been referenced in 1 publication.

    • Le Ribeuz H  et al. In vivo miR-138-5p inhibition alleviates monocrotaline-induced pulmonary hypertension and normalizes pulmonary KCNK3 and SLC45A3 expression. Respir Res 21:186 (2020). PubMed: 32678044

    Customer reviews and Q&As

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Prostein antibody [EPR4795(2)]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Prostate)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH9, Tris/EDTA based
    Permeabilization
    No
    Specification
    Prostate
    Blocking step
    Serum Free Protein Block (Dako) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
    Fixative
    10% NBF
    Read More

    Premier Laboratory

    Verified customer

    Submitted May 26 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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