Recombinant Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-SLC45A3 antibody [EPR4795(2)]
See all SLC45A3 primary antibodies -
Description
Rabbit monoclonal [EPR4795(2)] to SLC45A3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human SLC45A3 aa 400-500. The exact sequence is proprietary.
Database link: Q96JT2 -
Positive control
- WB: Human prostate cancer and normal tissue lysates and LnCaP cell lysates. IHC-P: Human benign prostatic adenocarcinoma and benign prostatic hyperplasia tissues.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as Prostein.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4795(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab137065 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 59 kDa). | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Tissue specificity
Prostate specific. Expressed in all prostatic glandular cells. Expressed both in normal and cancerous prostates. -
Sequence similarities
Belongs to the glycoside-pentoside-hexuronide (GPH) cation symporter transporter (TC 2.A.2) family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 85414 Human
- GenBank: BC050416 Human
- Omim: 605097 Human
- SwissProt: Q96JT2 Human
- Unigene: 278695 Human
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Alternative names
- IPCA 2 antibody
- IPCA 8 antibody
- IPCA6 antibody
see all
Images
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![Western blot - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)](https://www.abcam.com/ps/products/137/ab137065/Images/ab137065-151595-anti-prostein-antibody-epr47952-western-blot.jpg)
Western blot - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)All lanes : Anti-SLC45A3 antibody [EPR4795(2)] (ab137065) at 1/1000 dilution
Lane 1 : Human prostate cancer tissue lysate
Lane 2 : Human prostate tissue lysate
Lane 3 : LnCaP cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 59 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)](https://www.abcam.com/ps/products/137/ab137065/Images/ab137065-151594-anti-prostein-antibody-epr47952-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)Immunohistochemical analysis of paraffin-embedded Human benign prostatic adenocarcinoma tissue labelling SLC45A3 with as137065 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)](https://www.abcam.com/ps/products/137/ab137065/Images/ab137065-265104-anti-prostein-antibody-epr47952-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)This image is courtesy of an abreview submitted by Faye DohertyImmunohistochemical analysis of human prostate tissue sections labelling SLC45A3 with ab137065 at a dilution of 1/2000. The sections were fixed with 10% NBF. The secondary antibody used was HRP conjugated goat anti rabbit IgG. Antigen retrieval was heat mediated using Tris/EDTA pH 9.0.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)](https://www.abcam.com/ps/products/137/ab137065/Images/ab137065-151593-anti-prostein-antibody-epr47952-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)Immunohistochemical analysis of paraffin-embedded Human benign prostatic hyperplasia tissue labelling SLC45A3 with ab137065 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
References
ab137065 has not yet been referenced specifically in any publications.
