Recombinant
RabMAb

Recombinant Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)

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Overview

  • Product name

    Anti-SLC45A3 antibody [EPR4795(2)]
    See all SLC45A3 primary antibodies

  • Description

    Rabbit monoclonal [EPR4795(2)] to SLC45A3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    corresponding to Human SLC45A3 aa 400-500.
    Database link: Q96JT2

  • Positive control

    • Human prostate cancer and Human prostate tissue lysates and LnCaP cell lysates; Human benign prostatic adenocarcinoma and Human benign prostatic hyperplasia tissues

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as Prostein

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab137065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 59 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC or IP.

    • Target

    • Tissue specificity

      Prostate specific. Expressed in all prostatic glandular cells. Expressed both in normal and cancerous prostates.

    • Sequence similarities

      Belongs to the glycoside-pentoside-hexuronide (GPH) cation symporter transporter (TC 2.A.2) family.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • IPCA 2 antibody
        • IPCA 8 antibody
        • IPCA6 antibody
        • PCANAP2 antibody
        • PCANAP6 antibody
        • PCANAP8 antibody
        • Prostate cancer associated gene 2 antibody
        • Prostate cancer associated gene 6 antibody
        • Prostate cancer associated gene 8 antibody
        • Prostate cancer associated protein 2 antibody
        • Prostate cancer associated protein 6 antibody
        • Prostate cancer associated protein 8 antibody
        • Prostate cancer-associated protein 6 antibody
        • Prostein antibody
        • PRST antibody
        • S45A3_HUMAN antibody
        • Slc45a3 antibody
        • Solute carrier family 45 member 3 antibody
        see all

      Images

      • Western blot - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)
        Western blot - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)
        All lanes : Anti-SLC45A3 antibody [EPR4795(2)] (ab137065) at 1/1000 dilution

        Lane 1 : Human prostate cancer tissue lysate
        Lane 2 : Human prostate tissue lysate
        Lane 3 : LnCaP cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 59 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)

        Immunohistochemical analysis of paraffin-embedded Human benign prostatic adenocarcinoma tissue labelling SLC45A3 with as137065 at 1/100 dilution.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)This image is courtesy of an abreview submitted by Faye Doherty

        Immunohistochemical analysis of human prostate tissue sections labelling SLC45A3 with ab137065 at a dilution of 1/2000. The sections were fixed with 10% NBF. The secondary antibody used was HRP conjugated goat anti rabbit IgG. Antigen retrieval was heat mediated using Tris/EDTA pH 9.0.

        See Abreview

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)

        Immunohistochemical analysis of paraffin-embedded Human benign prostatic hyperplasia tissue labelling SLC45A3 with ab137065 at 1/100 dilution.

      References

      ab137065 has not yet been referenced specifically in any publications.

      Publishing research using ab137065? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Prostein antibody [EPR4795(2)]

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Prostate)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: pH9, Tris/EDTA based
      Permeabilization
      No
      Specification
      Prostate
      Blocking step
      Serum Free Protein Block (Dako) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
      Fixative
      10% NBF
      Read More

      Premier Laboratory

      Verified customer

      Submitted May 26 2016

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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