Recombinant
RabMAb

Recombinant Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)

Overview

  • Product name

    Anti-SLC45A3 antibody [EPR4795(2)]
    See all SLC45A3 primary antibodies
  • Description

    Rabbit monoclonal [EPR4795(2)] to SLC45A3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    corresponding to Human SLC45A3 aa 400-500.
    Database link: Q96JT2

  • Positive control

    • Human prostate cancer and Human prostate tissue lysates and LnCaP cell lysates; Human benign prostatic adenocarcinoma and Human benign prostatic hyperplasia tissues
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as Prostein

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab137065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 59 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC or IP.
  • Target

    • Tissue specificity

      Prostate specific. Expressed in all prostatic glandular cells. Expressed both in normal and cancerous prostates.
    • Sequence similarities

      Belongs to the glycoside-pentoside-hexuronide (GPH) cation symporter transporter (TC 2.A.2) family.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • IPCA 2 antibody
      • IPCA 8 antibody
      • IPCA6 antibody
      • PCANAP2 antibody
      • PCANAP6 antibody
      • PCANAP8 antibody
      • Prostate cancer associated gene 2 antibody
      • Prostate cancer associated gene 6 antibody
      • Prostate cancer associated gene 8 antibody
      • Prostate cancer associated protein 2 antibody
      • Prostate cancer associated protein 6 antibody
      • Prostate cancer associated protein 8 antibody
      • Prostate cancer-associated protein 6 antibody
      • Prostein antibody
      • PRST antibody
      • S45A3_HUMAN antibody
      • Slc45a3 antibody
      • Solute carrier family 45 member 3 antibody
      see all

    Images

    • All lanes : Anti-SLC45A3 antibody [EPR4795(2)] (ab137065) at 1/1000 dilution

      Lane 1 : Human prostate cancer tissue lysate
      Lane 2 : Human prostate tissue lysate
      Lane 3 : LnCaP cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 59 kDa

    • Immunohistochemical analysis of paraffin-embedded Human benign prostatic adenocarcinoma tissue labelling SLC45A3 with as137065 at 1/100 dilution.

    • Immunohistochemical analysis of human prostate tissue sections labelling SLC45A3 with ab137065 at a dilution of 1/2000. The sections were fixed with 10% NBF. The secondary antibody used was HRP conjugated goat anti rabbit IgG. Antigen retrieval was heat mediated using Tris/EDTA pH 9.0.

      See Abreview

    • Immunohistochemical analysis of paraffin-embedded Human benign prostatic hyperplasia tissue labelling SLC45A3 with ab137065 at 1/100 dilution.

    References

    ab137065 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Prostate)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH9, Tris/EDTA based
    Permeabilization
    No
    Specification
    Prostate
    Blocking step
    Serum Free Protein Block (Dako) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
    Fixative
    10% NBF

    Premier Laboratory

    Verified customer

    Submitted May 26 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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