Recombinant Anti-SLC45A3 antibody [EPR4795(2)] (ab137065)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4795(2)] to SLC45A3
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SLC45A3 antibody [EPR4795(2)]
See all SLC45A3 primary antibodies -
Description
Rabbit monoclonal [EPR4795(2)] to SLC45A3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human SLC45A3 aa 400-500. The exact sequence is proprietary.
Database link: Q96JT2 -
Positive control
- WB: Human prostate tissue lysate; IHC-P: Human benign prostatic adenocarcinoma, prostatic hyperplasia and prostate carcinoma tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4795(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137065 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 59 kDa).
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IHC-P | (1) |
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurifed use at 1/100 - 1/250 |
Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 59 kDa). |
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurifed use at 1/100 - 1/250 |
Target
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Tissue specificity
Prostate specific. Expressed in all prostatic glandular cells. Expressed both in normal and cancerous prostates. -
Sequence similarities
Belongs to the glycoside-pentoside-hexuronide (GPH) cation symporter transporter (TC 2.A.2) family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 85414 Human
- GenBank: BC050416 Human
- Omim: 605097 Human
- SwissProt: Q96JT2 Human
- Unigene: 278695 Human
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Alternative names
- IPCA 2 antibody
- IPCA 8 antibody
- IPCA6 antibody
see all
Images
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Anti-SLC45A3 antibody [EPR4795(2)] (ab137065) at 1/1000 dilution (Purified) + Human prostate lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 59 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?Blocking/Diluting buffer: 5% NFDM/TBST
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue sections labeling SLC45A3 with purified ab137065 at 1/1000 dilution (0.128 µg/mL). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemical analysis of paraffin-embedded Human prostatic adenocarcinoma tissue labelling SLC45A3 with as137065, unpurified, at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of human prostate tissue sections labelling SLC45A3 with ab137065, unpurified, at a dilution of 1/2000. The sections were fixed with 10% NBF. The secondary antibody used was HRP conjugated goat anti rabbit IgG. Antigen retrieval was heat mediated using Tris/EDTA pH 9.0.
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Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labelling SLC45A3 with ab137065, unpurified, at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab137065 has been referenced in 2 publications.
- Le Ribeuz H et al. In vivo miR-138-5p inhibition alleviates monocrotaline-induced pulmonary hypertension and normalizes pulmonary KCNK3 and SLC45A3 expression. Respir Res 21:186 (2020). PubMed: 32678044
- Ma X et al. Identification of a distinct luminal subgroup diagnosing and stratifying early stage prostate cancer by tissue-based single-cell RNA sequencing. Mol Cancer 19:147 (2020). PubMed: 33032611