Key features and details
- Rabbit polyclonal to SLC46A3
- Suitable for: WB, IHC-P, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SLC46A3 antibody
See all SLC46A3 primary antibodies
DescriptionRabbit polyclonal to SLC46A3
Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
- Jurkat cell lysate. Human kidney.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab62301 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2.5 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 52 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
|ELISA||Use at an assay dependent concentration.
Titre using peptide based assay: 1/62500.
RelevanceSLC46A3 (Solute carrier family 46 member 3) is a member of the major facilitator superfamily and SLC46A family. Its function remains unknown.
Cellular localizationMembrane; Multi-pass membrane protein.
- FKSG 16 antibody
- FKSG16 antibody
- FLJ42613 antibody
All lanes : SLC46A3 antibody (ab62301) at 2.5µg/ml (in 5% skim milk / PBS buffer)
Lane 1 : Molecular weight marker
Lane 2 : Jurkat cell lysate at 10 µg
All lanes : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 52 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
Additional bands at: 10 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SLC46A3 with ab62301 at 4-8µg/ml. Arrows indicate positively stained epithelial cells of the renal tubule. Magnification: 400X.
ab62301 has not yet been referenced specifically in any publications.