Overview

  • Product name

  • Description

    Rabbit polyclonal to SLC46A3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 of Human SLC46A3 (MKILFVEPAI FLSAFAMTLT GPLTTQYVYR RIWEETGNYT FSSDSNISEC)

  • Positive control

    • Jurkat cell lysate. Human kidney.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab62301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 52 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

Target

  • Relevance

    SLC46A3 (Solute carrier family 46 member 3) is a member of the major facilitator superfamily and SLC46A family. Its function remains unknown.
  • Cellular localization

    Membrane; Multi-pass membrane protein.
  • Database links

  • Alternative names

    • FKSG 16 antibody
    • FKSG16 antibody
    • FLJ42613 antibody
    • OTTHUMP00000018184 antibody
    • RP11 97E23.2 antibody
    • Solute carrier family 46 member 3 antibody
    see all

Images

  • All lanes : SLC46A3 antibody (ab62301) at 2.5µg/ml (in 5% skim milk / PBS buffer)

    Lane 1 : Molecular weight marker
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    All lanes : HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 52 kDa
    Observed band size: 44 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 10 kDa. We are unsure as to the identity of these extra bands.



    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SLC46A3 with ab62301 at 4-8µg/ml. Arrows indicate positively stained epithelial cells of the renal tubule. Magnification: 400X.

References

ab62301 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab62301.
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