Product nameAnti-SLC4A4 / NBC antibody
See all SLC4A4 / NBC primary antibodies
DescriptionRabbit polyclonal to SLC4A4 / NBC
Tested applicationsSuitable for: WB, IHC-Frmore details
Species reactivityReacts with: Rat
Synthetic peptide corresponding to Rat SLC4A4/ NBC (C terminal). Synthetic peptide derived from the C-terminus of rat renal splice variant.
Protein previously labeled as SLC4A4.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Constituent: 50% Glycerol
Our Abpromise guarantee covers the use of ab30322 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/2500. Predicted molecular weight: 120 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionElectrogenic sodium/bicarbonate cotransporter with a Na(+):HCO3(-) stoichiometry varying from 1:2 to 1:3. May regulate bicarbonate influx/efflux at the basolateral membrane of cells and regulate intracellular pH.
Tissue specificityIsoform 1 is expressed in pancreas and to a lower extent in heart, skeletal muscle, liver, parotid salivary glands, prostate, colon, stomach, thyroid, brain and spinal chord. Corneal endothelium cells express only isoform 1 (at protein level). Isoform 2 is specifically expressed in kidney at the level of proximal tubules.
Involvement in diseaseDefects in SLC4A4 are the cause of proximal renal tubular acidosis with ocular abnormalities (pRTA-OA) [MIM:604278]; also known as renal tubular acidosis II. Caused by an impairment of bicarbonate absorption in the proximal tubule, proximal renal tubular acidosis (pRTA) is characterized by a decreased renal HCO3(-) threshold. pRTA-OA is an extremely rare autosomal recessive syndrome characterized by short stature, profound pRTA, mental retardation, bilateral glaucoma, cataracts and bandkeratopathy.
Note=Loss of interaction with and stimulation by CA4 is the cause of retinitis pigmentosa type 17 (RP17).
Sequence similaritiesBelongs to the anion exchanger (TC 2.A.31) family.
modificationsPhosphorylation of Ser-1026 by PKA increases the binding of CA2 and changes the Na(+):HCO3(-) stoichiometry of the transporter from 3:1 to 2:1. Phosphorylation of Thr-49 regulates isoform 1 conductance.
N-glycosylation is not necessary for the transporter basic functions.
Cellular localizationBasolateral cell membrane.
- Information by UniProt
- DKFZp781H1314 antibody
- Electrogenic sodium bicarbonate cotransporter 1 antibody
- hhNMC antibody
This product has been referenced in:
- Yin J et al. Molecular Basis and Differentiation-Associated Alterations of Anion Secretion in Human Duodenal Enteroid Monolayers. Cell Mol Gastroenterol Hepatol 5:591-609 (2018). Read more (PubMed: 29930980) »
- Chinigarzadeh A et al. Genistein induces increase in fluid pH, Na+ and HCO3(-) concentration, SLC26A6 and SLC4A4 (NBCe1)-B expression in the uteri of ovariectomized rats. Int J Mol Sci 15:958-76 (2014). Read more (PubMed: 24434640) »