• Product name
  • Description
    Rabbit polyclonal to SLC4A4
  • Host species
  • Tested applications
    Suitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human, Recombinant fragment
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to C terminal residues of human SLC4A4



Our Abpromise guarantee covers the use of ab56215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 120 kDa. for 2 hours. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA Use at an assay dependent dilution.


  • Function
    Electrogenic sodium/bicarbonate cotransporter with a Na(+):HCO3(-) stoichiometry varying from 1:2 to 1:3. May regulate bicarbonate influx/efflux at the basolateral membrane of cells and regulate intracellular pH.
  • Tissue specificity
    Isoform 1 is expressed in pancreas and to a lower extent in heart, skeletal muscle, liver, parotid salivary glands, prostate, colon, stomach, thyroid, brain and spinal chord. Corneal endothelium cells express only isoform 1 (at protein level). Isoform 2 is specifically expressed in kidney at the level of proximal tubules.
  • Involvement in disease
    Defects in SLC4A4 are the cause of proximal renal tubular acidosis with ocular abnormalities (pRTA-OA) [MIM:604278]; also known as renal tubular acidosis II. Caused by an impairment of bicarbonate absorption in the proximal tubule, proximal renal tubular acidosis (pRTA) is characterized by a decreased renal HCO3(-) threshold. pRTA-OA is an extremely rare autosomal recessive syndrome characterized by short stature, profound pRTA, mental retardation, bilateral glaucoma, cataracts and bandkeratopathy.
    Note=Loss of interaction with and stimulation by CA4 is the cause of retinitis pigmentosa type 17 (RP17).
  • Sequence similarities
    Belongs to the anion exchanger (TC 2.A.31) family.
  • Post-translational
    Phosphorylation of Ser-1026 by PKA increases the binding of CA2 and changes the Na(+):HCO3(-) stoichiometry of the transporter from 3:1 to 2:1. Phosphorylation of Thr-49 regulates isoform 1 conductance.
    N-glycosylation is not necessary for the transporter basic functions.
  • Cellular localization
    Basolateral cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp781H1314 antibody
    • Electrogenic sodium bicarbonate cotransporter 1 antibody
    • hhNMC antibody
    • HNBC 1 antibody
    • HNBC1 antibody
    • kNBC 1 antibody
    • KNBC antibody
    • kNBC1 antibody
    • Na(+)/HCO3(-) cotransporter antibody
    • Na+HCO3- cotransporter 4 antibody
    • NBC 1 antibody
    • NBC 2 antibody
    • NBC1 antibody
    • NBC2 antibody
    • Nbc4 antibody
    • NBCE 1 antibody
    • NBCE1 antibody
    • OTTHUMP00000160355 antibody
    • OTTHUMP00000218884 antibody
    • OTTHUMP00000218885 antibody
    • PNBC antibody
    • S4A4_HUMAN antibody
    • SLC4A4 antibody
    • SLC4A5 antibody
    • Sodium bicarbonate cotransporter 1 (sodium bicarbonate cotransporter, kidney; sodium bicarbonate cotransporter, pancreas) antibody
    • Sodium bicarbonate cotransporter antibody
    • Sodium bicarbonate cotransporter kidney antibody
    • sodium bicarbonate cotransporter member 4 antibody
    • Sodium bicarbonate cotransporter pancreas antibody
    • Solute carrier family 4 member 4 antibody
    • Solute carrier family 4 sodium bicarbonate cotransporter member 4 antibody
    • Solute carrier family 4 sodium bicarbonate cotransporter member 5 antibody
    • Solute carrier family 4, sodium bicarbonate cotransporter, member 4, brain type antibody
    see all


  • ICC/IF image of ab56215 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56215, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:

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Customer reviews and Q&As

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I have made a short list of compatible antibodies for use withab56215 Anti-SLC4A4 antibody.These products are pre-absorbed to ensure minimum cross reactivity. Each product is ...

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Thank you for contacting us and your interest in our products. The immunogen sequence used to raise theab56215 Anti-SLC4A4 antibodyis proprietary information. However, in order to aid your research I am able to share it with you. Please do not share...

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