Anti-SLC4A4/NBC antibody (ab187511)
Key features and details
- Rabbit polyclonal to SLC4A4/NBC
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SLC4A4/NBC antibody
See all SLC4A4/NBC primary antibodies -
Description
Rabbit polyclonal to SLC4A4/NBC -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human SLC4A4/NBC aa 1-240 (N terminal).
Database link: Q9Y6R1 -
Positive control
- PC3 and A549 cell extracts.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187511 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 121 kDa.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 121 kDa. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Electrogenic sodium/bicarbonate cotransporter with a Na(+):HCO3(-) stoichiometry varying from 1:2 to 1:3. May regulate bicarbonate influx/efflux at the basolateral membrane of cells and regulate intracellular pH. -
Tissue specificity
Isoform 1 is expressed in pancreas and to a lower extent in heart, skeletal muscle, liver, parotid salivary glands, prostate, colon, stomach, thyroid, brain and spinal chord. Corneal endothelium cells express only isoform 1 (at protein level). Isoform 2 is specifically expressed in kidney at the level of proximal tubules. -
Involvement in disease
Defects in SLC4A4 are the cause of proximal renal tubular acidosis with ocular abnormalities (pRTA-OA) [MIM:604278]; also known as renal tubular acidosis II. Caused by an impairment of bicarbonate absorption in the proximal tubule, proximal renal tubular acidosis (pRTA) is characterized by a decreased renal HCO3(-) threshold. pRTA-OA is an extremely rare autosomal recessive syndrome characterized by short stature, profound pRTA, mental retardation, bilateral glaucoma, cataracts and bandkeratopathy.
Note=Loss of interaction with and stimulation by CA4 is the cause of retinitis pigmentosa type 17 (RP17). -
Sequence similarities
Belongs to the anion exchanger (TC 2.A.31) family. -
Post-translational
modificationsPhosphorylation of Ser-1026 by PKA increases the binding of CA2 and changes the Na(+):HCO3(-) stoichiometry of the transporter from 3:1 to 2:1. Phosphorylation of Thr-49 regulates isoform 1 conductance.
N-glycosylation is not necessary for the transporter basic functions. -
Cellular localization
Basolateral cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 8671 Human
- Entrez Gene: 54403 Mouse
- Entrez Gene: 84484 Rat
- Omim: 603345 Human
- SwissProt: Q9Y6R1 Human
- SwissProt: O88343 Mouse
- SwissProt: Q9JI66 Rat
- Unigene: 5462 Human
see all -
Alternative names
- DKFZp781H1314 antibody
- Electrogenic sodium bicarbonate cotransporter 1 antibody
- hhNMC antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat lung tissue labelling SLC4A4/NBC with ab187511 at 1/200. Magnification: 400x.
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All lanes : Anti-SLC4A4/NBC antibody (ab187511) at 1/500 dilution
Lane 1 : PC3 cell extract
Lane 2 : A549 cell extract
Developed using the ECL technique.
Predicted band size: 121 kDa -
Immunohistochemical analysis of paraffin embedded mouse kidney tissue labeling SLC4A4/NBC with ab187511 at 1:100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue labelling SLC4A4/NBC with ab187511 at 1/200. Magnification: 400x.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver cancer tissue labelling SLC4A4/NBC with ab187511 at 1/200. Magnification: 400x.
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Immunohistochemical analysis of paraffin embedded rat kidney tissue labeling SLC4A4/NBC with ab187511 at 1:100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab187511 has been referenced in 10 publications.
- Cappellesso F et al. Targeting the bicarbonate transporter SLC4A4 overcomes immunosuppression and immunotherapy resistance in pancreatic cancer. Nat Cancer 3:1464-1483 (2022). PubMed: 36522548
- Huang F et al. SLC34A2 Up-regulation And SLC4A4 Down-regulation Correlates With Invasion, Metastasis, And The MAPK Signaling Pathway In Papillary Thyroid Carcinomas. J Cancer 12:5439-5453 (2021). PubMed: 34405007
- Liang Y et al. Identification and Validation of a Novel Inflammatory Response-Related Gene Signature for the Prognosis of Colon Cancer. J Inflamm Res 14:3809-3821 (2021). PubMed: 34408464
- Jo J et al. Regional heterogeneity of astrocyte morphogenesis dictated by the formin protein, Daam2, modifies circuit function. EMBO Rep 22:e53200 (2021). PubMed: 34633730
- Bignon Y et al. Defective bicarbonate reabsorption in Kir4.2 potassium channel deficient mice impairs acid-base balance and ammonia excretion. Kidney Int 97:304-315 (2020). PubMed: 31870500
- Virreira M et al. Expression, Localization, and Regulation of the Sodium Bicarbonate Cotransporter NBCe1 in the Thyroid. Thyroid N/A:N/A (2019). PubMed: 30526387
- Hwang S et al. Drug Repurposing as an Antitumor Agent: Disulfiram-Mediated Carbonic Anhydrase 12 and Anion Exchanger 2 Modulation to Inhibit Cancer Cell Migration. Molecules 24:N/A (2019). PubMed: 31546841
- Abramicheva PA et al. Molecular Targets of Natriuretic Action of Prolactin in the Rat Model of Cholestasis of Pregnancy. Bull Exp Biol Med 168:219-223 (2019). PubMed: 31776946
- Dongre A et al. Cathepsin K in Lymphangioleiomyomatosis: LAM Cell-Fibroblast Interactions Enhance Protease Activity by Extracellular Acidification. Am J Pathol 187:1750-1762 (2017). PubMed: 28623674
- Foulke-Abel J et al. Human Enteroids as a Model of Upper Small Intestinal Ion Transport Physiology and Pathophysiology. Gastroenterology 150:638-649.e8 (2016). PubMed: 26677983