Key features and details
- Rabbit polyclonal to SLC4A4/NBC
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-SLC4A4/NBC antibody
See all SLC4A4/NBC primary antibodies
DescriptionRabbit polyclonal to SLC4A4/NBC
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment corresponding to Human SLC4A4/NBC aa 1-240 (N terminal).
Database link: Q9Y6R1
- PC3 and A549 cell extracts.
Protein previously labeled as SLC4A4.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab187511 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 121 kDa.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionElectrogenic sodium/bicarbonate cotransporter with a Na(+):HCO3(-) stoichiometry varying from 1:2 to 1:3. May regulate bicarbonate influx/efflux at the basolateral membrane of cells and regulate intracellular pH.
Tissue specificityIsoform 1 is expressed in pancreas and to a lower extent in heart, skeletal muscle, liver, parotid salivary glands, prostate, colon, stomach, thyroid, brain and spinal chord. Corneal endothelium cells express only isoform 1 (at protein level). Isoform 2 is specifically expressed in kidney at the level of proximal tubules.
Involvement in diseaseDefects in SLC4A4 are the cause of proximal renal tubular acidosis with ocular abnormalities (pRTA-OA) [MIM:604278]; also known as renal tubular acidosis II. Caused by an impairment of bicarbonate absorption in the proximal tubule, proximal renal tubular acidosis (pRTA) is characterized by a decreased renal HCO3(-) threshold. pRTA-OA is an extremely rare autosomal recessive syndrome characterized by short stature, profound pRTA, mental retardation, bilateral glaucoma, cataracts and bandkeratopathy.
Note=Loss of interaction with and stimulation by CA4 is the cause of retinitis pigmentosa type 17 (RP17).
Sequence similaritiesBelongs to the anion exchanger (TC 2.A.31) family.
modificationsPhosphorylation of Ser-1026 by PKA increases the binding of CA2 and changes the Na(+):HCO3(-) stoichiometry of the transporter from 3:1 to 2:1. Phosphorylation of Thr-49 regulates isoform 1 conductance.
N-glycosylation is not necessary for the transporter basic functions.
Cellular localizationBasolateral cell membrane.
- Information by UniProt
- DKFZp781H1314 antibody
- Electrogenic sodium bicarbonate cotransporter 1 antibody
- hhNMC antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat lung tissue labelling SLC4A4/NBC with ab187511 at 1/200. Magnification: 400x.
All lanes : Anti-SLC4A4/NBC antibody (ab187511) at 1/500 dilution
Lane 1 : PC3 cell extract
Lane 2 : A549 cell extract
Developed using the ECL technique.
Predicted band size: 121 kDa
Immunohistochemical analysis of paraffin embedded mouse kidney tissue labeling SLC4A4/NBC with ab187511 at 1:100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue labelling SLC4A4/NBC with ab187511 at 1/200. Magnification: 400x.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver cancer tissue labelling SLC4A4/NBC with ab187511 at 1/200. Magnification: 400x.
Immunohistochemical analysis of paraffin embedded rat kidney tissue labeling SLC4A4/NBC with ab187511 at 1:100 dilution.
ab187511 has been referenced in 4 publications.
- Virreira M et al. Expression, Localization, and Regulation of the Sodium Bicarbonate Cotransporter NBCe1 in the Thyroid. Thyroid N/A:N/A (2019). PubMed: 30526387
- Hwang S et al. Drug Repurposing as an Antitumor Agent: Disulfiram-Mediated Carbonic Anhydrase 12 and Anion Exchanger 2 Modulation to Inhibit Cancer Cell Migration. Molecules 24:N/A (2019). PubMed: 31546841
- Dongre A et al. Cathepsin K in Lymphangioleiomyomatosis: LAM Cell-Fibroblast Interactions Enhance Protease Activity by Extracellular Acidification. Am J Pathol 187:1750-1762 (2017). PubMed: 28623674
- Foulke-Abel J et al. Human Enteroids as a Model of Upper Small Intestinal Ion Transport Physiology and Pathophysiology. Gastroenterology 150:638-649.e8 (2016). PubMed: 26677983