Overview

  • Product name

  • Description

    Rabbit polyclonal to SLC4A4/NBC
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human SLC4A4/NBC aa 1-240 (N terminal).
    Database link: Q9Y6R1

  • Positive control

    • PC3 and A549 cell extracts.
  • General notes

    Protein previously labeled as SLC4A4.

Properties

Applications

Our Abpromise guarantee covers the use of ab187511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 121 kDa.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Electrogenic sodium/bicarbonate cotransporter with a Na(+):HCO3(-) stoichiometry varying from 1:2 to 1:3. May regulate bicarbonate influx/efflux at the basolateral membrane of cells and regulate intracellular pH.
  • Tissue specificity

    Isoform 1 is expressed in pancreas and to a lower extent in heart, skeletal muscle, liver, parotid salivary glands, prostate, colon, stomach, thyroid, brain and spinal chord. Corneal endothelium cells express only isoform 1 (at protein level). Isoform 2 is specifically expressed in kidney at the level of proximal tubules.
  • Involvement in disease

    Defects in SLC4A4 are the cause of proximal renal tubular acidosis with ocular abnormalities (pRTA-OA) [MIM:604278]; also known as renal tubular acidosis II. Caused by an impairment of bicarbonate absorption in the proximal tubule, proximal renal tubular acidosis (pRTA) is characterized by a decreased renal HCO3(-) threshold. pRTA-OA is an extremely rare autosomal recessive syndrome characterized by short stature, profound pRTA, mental retardation, bilateral glaucoma, cataracts and bandkeratopathy.
    Note=Loss of interaction with and stimulation by CA4 is the cause of retinitis pigmentosa type 17 (RP17).
  • Sequence similarities

    Belongs to the anion exchanger (TC 2.A.31) family.
  • Post-translational
    modifications

    Phosphorylation of Ser-1026 by PKA increases the binding of CA2 and changes the Na(+):HCO3(-) stoichiometry of the transporter from 3:1 to 2:1. Phosphorylation of Thr-49 regulates isoform 1 conductance.
    N-glycosylation is not necessary for the transporter basic functions.
  • Cellular localization

    Basolateral cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp781H1314 antibody
    • Electrogenic sodium bicarbonate cotransporter 1 antibody
    • hhNMC antibody
    • HNBC 1 antibody
    • HNBC1 antibody
    • kNBC 1 antibody
    • KNBC antibody
    • kNBC1 antibody
    • Na(+)/HCO3(-) cotransporter antibody
    • Na+HCO3- cotransporter 4 antibody
    • NBC 1 antibody
    • NBC 2 antibody
    • NBC1 antibody
    • NBC2 antibody
    • Nbc4 antibody
    • NBCE 1 antibody
    • NBCE1 antibody
    • OTTHUMP00000160355 antibody
    • OTTHUMP00000218884 antibody
    • OTTHUMP00000218885 antibody
    • PNBC antibody
    • S4A4_HUMAN antibody
    • SLC4A4 antibody
    • SLC4A5 antibody
    • Sodium bicarbonate cotransporter 1 (sodium bicarbonate cotransporter, kidney; sodium bicarbonate cotransporter, pancreas) antibody
    • Sodium bicarbonate cotransporter antibody
    • Sodium bicarbonate cotransporter kidney antibody
    • sodium bicarbonate cotransporter member 4 antibody
    • Sodium bicarbonate cotransporter pancreas antibody
    • Solute carrier family 4 member 4 antibody
    • Solute carrier family 4 sodium bicarbonate cotransporter member 4 antibody
    • Solute carrier family 4 sodium bicarbonate cotransporter member 5 antibody
    • Solute carrier family 4, sodium bicarbonate cotransporter, member 4, brain type antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat lung tissue labelling SLC4A4/NBC with ab187511 at 1/200. Magnification: 400x.

  • All lanes : Anti-SLC4A4/NBC antibody (ab187511) at 1/500 dilution

    Lane 1 : PC3 cell extract
    Lane 2 : A549 cell extract

    Developed using the ECL technique.

    Predicted band size: 121 kDa

  • Immunohistochemical analysis of paraffin embedded mouse kidney tissue labeling SLC4A4/NBC with ab187511 at 1:100 dilution. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue labelling SLC4A4/NBC with ab187511 at 1/200. Magnification: 400x.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver cancer tissue labelling SLC4A4/NBC with ab187511 at 1/200. Magnification: 400x.

  • Immunohistochemical analysis of paraffin embedded rat kidney tissue labeling SLC4A4/NBC with ab187511 at 1:100 dilution. 

References

This product has been referenced in:

  • Virreira M  et al. Expression, Localization, and Regulation of the Sodium Bicarbonate Cotransporter NBCe1 in the Thyroid. Thyroid N/A:N/A (2019). Read more (PubMed: 30526387) »
  • Foulke-Abel J  et al. Human Enteroids as a Model of Upper Small Intestinal Ion Transport Physiology and Pathophysiology. Gastroenterology 150:638-649.e8 (2016). Read more (PubMed: 26677983) »
See all 2 Publications for this product

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