Key features and details
- Rabbit polyclonal to SLC4A4/NBC
- Suitable for: WB, IHC-Fr
- Reacts with: Rat
- Isotype: IgG
Product nameAnti-SLC4A4/NBC antibody
See all SLC4A4/NBC primary antibodies
DescriptionRabbit polyclonal to SLC4A4/NBC
Tested applicationsSuitable for: WB, IHC-Frmore details
Species reactivityReacts with: Rat
Synthetic peptide corresponding to Rat SLC4A4/NBC (N terminal).
Protein previously labeled as SLC4A4.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Constituent: 50% Glycerol
Concentration information loading...
Our Abpromise guarantee covers the use of ab30323 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/2500. Predicted molecular weight: 120 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionElectrogenic sodium/bicarbonate cotransporter with a Na(+):HCO3(-) stoichiometry varying from 1:2 to 1:3. May regulate bicarbonate influx/efflux at the basolateral membrane of cells and regulate intracellular pH.
Tissue specificityIsoform 1 is expressed in pancreas and to a lower extent in heart, skeletal muscle, liver, parotid salivary glands, prostate, colon, stomach, thyroid, brain and spinal chord. Corneal endothelium cells express only isoform 1 (at protein level). Isoform 2 is specifically expressed in kidney at the level of proximal tubules.
Involvement in diseaseDefects in SLC4A4 are the cause of proximal renal tubular acidosis with ocular abnormalities (pRTA-OA) [MIM:604278]; also known as renal tubular acidosis II. Caused by an impairment of bicarbonate absorption in the proximal tubule, proximal renal tubular acidosis (pRTA) is characterized by a decreased renal HCO3(-) threshold. pRTA-OA is an extremely rare autosomal recessive syndrome characterized by short stature, profound pRTA, mental retardation, bilateral glaucoma, cataracts and bandkeratopathy.
Note=Loss of interaction with and stimulation by CA4 is the cause of retinitis pigmentosa type 17 (RP17).
Sequence similaritiesBelongs to the anion exchanger (TC 2.A.31) family.
modificationsPhosphorylation of Ser-1026 by PKA increases the binding of CA2 and changes the Na(+):HCO3(-) stoichiometry of the transporter from 3:1 to 2:1. Phosphorylation of Thr-49 regulates isoform 1 conductance.
N-glycosylation is not necessary for the transporter basic functions.
Cellular localizationBasolateral cell membrane.
- Information by UniProt
- DKFZp781H1314 antibody
- Electrogenic sodium bicarbonate cotransporter 1 antibody
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ab30323 has been referenced in 1 publication.
- Khakipoor S et al. Functional expression of electrogenic sodium bicarbonate cotransporter 1 (NBCe1) in mouse cortical astrocytes is dependent on S255-257 and regulated by mTOR. Glia 67:2264-2278 (2019). PubMed: 31318482