Overview

  • Product name

  • Description

    Rabbit polyclonal to SLC4A4/NBC
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity

    Reacts with: Human, Recombinant fragment
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human SLC4A4/NBC (C terminal).
    Database link: Q9Y6R1

  • General notes

    Protein previously labeled as SLC4A4.

Properties

Applications

Our Abpromise guarantee covers the use of ab56215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 120 kDa. for 2 hours. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA Use at an assay dependent dilution.

Target

  • Function

    Electrogenic sodium/bicarbonate cotransporter with a Na(+):HCO3(-) stoichiometry varying from 1:2 to 1:3. May regulate bicarbonate influx/efflux at the basolateral membrane of cells and regulate intracellular pH.
  • Tissue specificity

    Isoform 1 is expressed in pancreas and to a lower extent in heart, skeletal muscle, liver, parotid salivary glands, prostate, colon, stomach, thyroid, brain and spinal chord. Corneal endothelium cells express only isoform 1 (at protein level). Isoform 2 is specifically expressed in kidney at the level of proximal tubules.
  • Involvement in disease

    Defects in SLC4A4 are the cause of proximal renal tubular acidosis with ocular abnormalities (pRTA-OA) [MIM:604278]; also known as renal tubular acidosis II. Caused by an impairment of bicarbonate absorption in the proximal tubule, proximal renal tubular acidosis (pRTA) is characterized by a decreased renal HCO3(-) threshold. pRTA-OA is an extremely rare autosomal recessive syndrome characterized by short stature, profound pRTA, mental retardation, bilateral glaucoma, cataracts and bandkeratopathy.
    Note=Loss of interaction with and stimulation by CA4 is the cause of retinitis pigmentosa type 17 (RP17).
  • Sequence similarities

    Belongs to the anion exchanger (TC 2.A.31) family.
  • Post-translational
    modifications

    Phosphorylation of Ser-1026 by PKA increases the binding of CA2 and changes the Na(+):HCO3(-) stoichiometry of the transporter from 3:1 to 2:1. Phosphorylation of Thr-49 regulates isoform 1 conductance.
    N-glycosylation is not necessary for the transporter basic functions.
  • Cellular localization

    Basolateral cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp781H1314 antibody
    • Electrogenic sodium bicarbonate cotransporter 1 antibody
    • hhNMC antibody
    • HNBC 1 antibody
    • HNBC1 antibody
    • kNBC 1 antibody
    • KNBC antibody
    • kNBC1 antibody
    • Na(+)/HCO3(-) cotransporter antibody
    • Na+HCO3- cotransporter 4 antibody
    • NBC 1 antibody
    • NBC 2 antibody
    • NBC1 antibody
    • NBC2 antibody
    • Nbc4 antibody
    • NBCE 1 antibody
    • NBCE1 antibody
    • OTTHUMP00000160355 antibody
    • OTTHUMP00000218884 antibody
    • OTTHUMP00000218885 antibody
    • PNBC antibody
    • S4A4_HUMAN antibody
    • SLC4A4 antibody
    • SLC4A5 antibody
    • Sodium bicarbonate cotransporter 1 (sodium bicarbonate cotransporter, kidney; sodium bicarbonate cotransporter, pancreas) antibody
    • Sodium bicarbonate cotransporter antibody
    • Sodium bicarbonate cotransporter kidney antibody
    • sodium bicarbonate cotransporter member 4 antibody
    • Sodium bicarbonate cotransporter pancreas antibody
    • Solute carrier family 4 member 4 antibody
    • Solute carrier family 4 sodium bicarbonate cotransporter member 4 antibody
    • Solute carrier family 4 sodium bicarbonate cotransporter member 5 antibody
    • Solute carrier family 4, sodium bicarbonate cotransporter, member 4, brain type antibody
    see all

Images

  • ICC/IF image of ab56215 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56215, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Q&A

Answer

Thank you for contacting us.

I show that we currently have stock of this product in our UK and Japan offices but not in the US. Transfer from one of these offices usually adds a day or two. I show an estimated delivery of ab56215 on 11 October.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
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Question
Answer

Thank you for contacting us today regarding secondary antibodies.

I have made a short list of compatible antibodies for use withab56215 Anti-SLC4A4 antibody.These products are pre-absorbed to ensure minimum cross reactivity. Each product is conjugated a different fluorochome so that you may chose the one which best fits your experiments. Please let me know if you have any questions.

AMCA:https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-AMCA-ab123435.html

PE:https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-PE-pre-adsorbed-ab72465.html

DyLight 594:https://www.abcam.com/Donkey-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-DyLight-594-pre-adsorbed-ab98500.html

Texas Red:https://www.abcam.com/Rabbit-IgG-secondary-antibody-H-L-Pre-Adsorbed-ab7088.html

APC:https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-HL-Allophycocyanin-pre-adsorbed-ab130805.html

If testing live cells we also recommend cell viability controls such as 7-AAD orPropidium Iodide.Propidium Iodide (PI) binds to double-stranded DNA. PI cannot cross intact plasma membrane and therefore will only be present in DNA of cells where the plasma membrane has been compromised/ permeabilized. We do carry PI and offer a 7-AADCell-Mediated Cytotoxicity Assay Kit.

Propidium Iodide: https://www.abcam.com/Propidium-Iodide-ab14083.html

7-AAD Kit:https://www.abcam.com/7-AADCFSE-Cytotoxicity-Assay-Kit-ab133073.html



In reviewing the datasheet for this product I did note that it has not been tested in Flow Cytometry. As such we cannot guarantee ab56212 in that application. However by participating in our AbTrial program, you can now use our products in an untested application or species without financial risk.



Simply follow these easy steps below to take part in our AbTrial Program:

1. Reply to this e-mail to let me know that you would like to proceed and test this product in Flow Cytometry.

2. I will email you aninactivepersonal discount code for the value of the product.

3. Purchase and test the product at regular price.

4. https://www.abcam.com/abreviews with you results noting your AbTrial discount code in the additional notes section.

5. Once the Abreview is submitted, the discount code will becomeactive.

6. Apply your discount code on your next order to receive that value off.

More information including the terms and conditions about this program may be found at: https://www.abcam.com/AbTrial

I hope this information has been of help to you. If you have any further questions, please do not hesitate to ask.

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Question
Answer

Thank you for contacting us and your interest in our products. The immunogen sequence used to raise theab56215 Anti-SLC4A4 antibodyis proprietary information. However, in order to aid your research I am able to share it with you. Please do not share or publish this information. It is strictly for your own use. The peptide sequence used is as follows: Abcam also carries secondary antibodies for use in Flow Cytometry and Direct Conjugation Kits. Our EasyLink kits providea simple and quick process to conjugate your primary antibodies with FITC. Ab102885 EasyLink FITC Conjugation Kit can be found at the following link: https://www.abcam.com/easylink-fitc-conjugation-kit-3-x-10%C2%B5g-fitc-ab102885.html OurGoat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed can be found at: https://www.abcam.com/goat-polyclonal-secondary-antibody-to-rabbit-igg-h-l-dylight-488-pre-adsorbed-ab96899.html For a limited time we are offering our Cell Imaging Book with any secondary antibody order. Just include the codeCELL-TBVCQ when ordering. More information about this promotion and our Cell Imaging Book can be found at: https://www.abcam.com/index.html?pageconfig=resource&rid=14705 I hope this information has been of help to you. If you have any further questions, please do not hesitate to ask.

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