Key features and details
- Rabbit polyclonal to SLC4A5
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SLC4A5 antibody
DescriptionRabbit polyclonal to SLC4A5
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human SLC4A5 aa 1071-1137.
Database link: Q9BY07
- ICC/IF: HepG2 cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity greater than 95%.
Our Abpromise guarantee covers the use of ab237042 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
FunctionMediates sodium- and bicarbonate-dependent electrogenic sodium bicarbonate cotransport, with a Na(+):HCO3(-) stoichiometry of 2:1. May have a housekeeping function in regulating the pH of tissues in which it is expressed. May play a role in mediating Na(+):HCO3(-) cotransport in hepatocytes and intrahepatic cholangiocytes. Also may be important in protecting the renal paranchyma from alterations in urine pH.
Tissue specificityHighest expression observed in liver, spleen and testis; moderate expression in the choroid plexus, hippocampus, cerebrum and cerebellum of brain, and in kidney cortex and kidney medulla. Also observed in heart, pancreas, muscle, lung, placenta, stomach and small intestine. Weakest expression seen in peripheral blood lymphocytes, colon, duodenum, jejunum, ileum and skeletal muscle.
Sequence similaritiesBelongs to the anion exchanger (TC 2.A.31) family.
Cellular localizationApical cell membrane. Expressed in the apical plasma membrane domain of a subset of collecting ducts in the renal medulla.
- Information by UniProt
- Electrogenic sodium bicarbonate cotransporter 4 antibody
- MGC129662 antibody
- NBC4 antibody
4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells stained for SLC4A5 (green) using ab237042 at 1/133 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI. Blocked in 10% normal Goat Serum.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237042 has not yet been referenced specifically in any publications.