Key features and details
- Rabbit polyclonal to SLC4A7/NBCn1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-SLC4A7/NBCn1 antibody
See all SLC4A7/NBCn1 primary antibodies
DescriptionRabbit polyclonal to SLC4A7/NBCn1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Monkey
- WB: A549, RAW 264.7 and PC-12 whole cell lysates. IHC-P: Human pancreas and kidney tissues.
This product was previously labelled as SLC4A7
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230848 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 136 kDa.|
FunctionElectroneutral sodium- and bicarbonate-dependent cotransporter with a Na(+):HCO3(-) 1:1 stoichiometry. Regulates intracellular pH and may play a role in bicarbonate salvage in secretory epithelia. May also have an associated sodium channel activity.
Tissue specificityHighly expressed in testis and spleen. Also expressed in retina, colon, small intestine, ovary, thymus, prostate, muscle, heart and kidney. Isoform 1 is expressed in skeletal muscle and heart muscle.
Sequence similaritiesBelongs to the anion exchanger (TC 2.A.31) family.
DomainThe PDZ-binding motif mediates interaction with the CFTR, SLC9A3R1/EBP50 complex and probably with USH1C.
Cellular localizationBasolateral cell membrane. Apical cell membrane. Cell projection, stereocilium. Also described at the apical cell membrane. Localizes to the stereocilia of cochlear outer hair cells and to the lateral membrane of cochlear inner hair cells (By similarity).
- Information by UniProt
- Bicarbonate transporter antibody
- BT antibody
- Electroneutral Na/HCO(3) cotransporter antibody
Formalin-fixed, paraffin-embedded human pancreas tissue stained for SLC4A7/NBCn1 using ab230848 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human kidney tissue stained for SLC4A7/NBCn1 using ab230848 at 1/100 dilution in immunohistochemical analysis.
All lanes : Anti-SLC4A7/NBCn1 antibody (ab230848) at 1/500 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Developed using the ECL technique.
Predicted band size: 136 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230848 has not yet been referenced specifically in any publications.