Key features and details
- Rabbit polyclonal to SLC51B
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SLC51B antibody
See all SLC51B primary antibodies
DescriptionRabbit polyclonal to SLC51B
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
- WB: Caco-2 cell lysate. IHC-P: Human small intestine, testis and kidney tissue.
This product was previously labelled as OST-beta
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121285 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionEssential component of the Ost-alpha/Ost-beta complex, a heterodimer that acts as the intestinal basolateral transporter responsible for bile acid export from enterocytes into portal blood. Efficiently transports the major species of bile acids.
Tissue specificityWidely expressed with a high expression in ileum. Expressed in testis, colon, liver, small intestine, kidney, ovary and adrenal gland; and at low levels in heart, lung, brain, pituitary, thyroid gland, uterus, prostate, mammary gland and fat.
Sequence similaritiesBelongs to the OST-beta family.
Cellular localizationCell membrane. Mainly restricted to the lateral and basal membranes of ileal enterocytes.
- Information by UniProt
- Organic solute transporter subunit beta antibody
- OST-beta antibody
- OSTB antibody
- OSTB_HUMAN antibody
Immunohistochemical analysis of human testis tissue labeling OST beta with ab121285 at 1/50 dilution.
All lanes : Anti-SLC51B antibody (ab121285) at 0.4 µg/ml
Lane 1 : Caco-2 (Human colorectal adenocarcinoma cell line) cell lysate
Lane 2 : U-2 OS (Human bone osteosarcoma epithelial cell line) cell lysate
Observed band size: 14 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of human kidney tissue labeling OST beta with ab121285 at 1/50 dilution.
Immunohistochemical analysis of human liver tissue labeling OST beta with ab121285 at 1/50 dilution. This showes no positivity as expected.
Immunohistochemical analysis of human small intestine tissue labeling OST beta with ab121285 at 1/50 dilution.
ab121285 has been referenced in 1 publication.
- Wang C et al. Morin protects glucocorticoid-induced osteoporosis through regulating the mitogen-activated protein kinase signaling pathway. J Nat Med 72:929-936 (2018). PubMed: 30043216