Anti-SLC6A15 antibody - N-terminal (ab230890)

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Western blot - Anti-SLC6A15 antibody - N-terminal (ab230890)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A15 antibody - N-terminal (ab230890)

Key features and details

  • Rabbit polyclonal to SLC6A15 - N-terminal
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-SLC6A15 antibody - N-terminal
    See all SLC6A15 primary antibodies

  • Description

    Rabbit polyclonal to SLC6A15 - N-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Monkey

  • Immunogen

    Synthetic peptide corresponding to Human SLC6A15 (N terminal) conjugated to keyhole limpet haemocyanin.
    Database link: Q9H2J7

  • Positive control

    • WB: HEK-293T, mouse and rat brain whole cell lysate. IHC-P: Human brain cortex tissue.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab230890 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 82 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Functions as a sodium-dependent neutral amino acid transporter. Exhibits preference for the branched-chain amino acids, particulary leucine, valine and isoleucine and methionine. Mediates the saturable, pH-sensitive and electrogenic cotransport of proline and sodium ions with a stoichiometry of 1:1. May have a role as transporter for neurotransmitter precursors into neurons. In contrast to other members of the neurotransmitter transporter family, does not appear to be chloride-dependent.

  • Tissue specificity

    Almost exclusively expressed in the brain.

  • Sequence similarities

    Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A15 subfamily.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • hv7 3 antibody
    • MGC87066 antibody
    • NTT73 antibody
    • Orphan sodium and chloride dependent neurotransmitter transporter NTT73 antibody
    • Orphan transporter v7 3 antibody
    • S6A15_HUMAN antibody
    • SBAT1 antibody
    • SLC6A15 antibody
    • sodium- and chloride-dependent neurotransmitter transporter NTT73 antibody
    • Sodium-coupled branched-chain amino-acid transporter 1 antibody
    • Sodium-dependent neutral amino acid transporter B(0)AT2 antibody
    • Solute carrier family 6 (neutral amino acid transporter) member 15 antibody
    • Solute carrier family 6 member 15 antibody
    • Transporter v7-3 antibody
    • V7 3 antibody
    see all

Images

  • Western blot - Anti-SLC6A15 antibody - N-terminal (ab230890)
    Western blot - Anti-SLC6A15 antibody - N-terminal (ab230890)
    All lanes : Anti-SLC6A15 antibody - N-terminal (ab230890) at 1/500 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : Mouse brain whole cell lysate
    Lane 3 : Rat brain whole cell lysate

    Developed using the ECL technique.

    Predicted band size: 82 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A15 antibody - N-terminal (ab230890)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A15 antibody - N-terminal (ab230890)

    Formalin-fixed, paraffin-embedded human brain cortex tissue stained for SLC6A15 with ab230890 at a 1/100 dilution in immunohistochemical analysis.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab230890? Please let us know so that we can cite the reference in this datasheet.

ab230890 has not yet been referenced specifically in any publications.

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