RecombinantRabMAb

Recombinant Anti-SLC6A19 antibody [EPR14154(B)] (ab180516)

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Western blot - Anti-SLC6A19 antibody [EPR14154(B)] (ab180516)
  • Immunocytochemistry - Anti-SLC6A19 antibody [EPR14154(B)] (ab180516)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A19 antibody [EPR14154(B)] (ab180516)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A19 antibody [EPR14154(B)] (ab180516)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A19 antibody [EPR14154(B)] (ab180516)
  • ELISA - Anti-SLC6A19 antibody [EPR14154(B)] (ab180516)
  • Anti-SLC6A19 antibody [EPR14154(B)] (ab180516)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14154(B)] to SLC6A19
  • Suitable for: ICC, ELISA, WB, IHC-P
  • Reacts with: Mouse, Rat, Human, Recombinant fragment

Overview

  • Product name

    Anti-SLC6A19 antibody [EPR14154(B)]
    See all SLC6A19 primary antibodies

  • Description

    Rabbit monoclonal [EPR14154(B)] to SLC6A19

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ELISA
    Recombinant fragment
    ICC
    Human
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human SLC6A19 aa 350-450 (internal sequence). The exact sequence is proprietary.
    Database link: Q695T7

  • Positive control

    • IHC: Human and mouse colon tissues. Rat kidney tissue. ICC: HeLa cells; WB: Caco-2, HeLa whole cell lysates, Human and mouse kidney lysates, mouse and rat brain lysates and mouse and rat heart lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR14154(B)

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab180516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ELISA
Recombinant fragment
ICC
Human
IHC-P
Mouse
Rat
Human
WB
Mouse
Rat
Human
Application Abreviews Notes
ICC
1/50.

For unpurified use at 1/500.
ELISA
Use at an assay dependent concentration.
WB
1/1000. Predicted molecular weight: 71 kDa.

For unpurified use at 1/1000 - 1/10000.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/50 - 1/100.
Notes
ICC
1/50.

For unpurified use at 1/500.
ELISA
Use at an assay dependent concentration.
WB
1/1000. Predicted molecular weight: 71 kDa.

For unpurified use at 1/1000 - 1/10000.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/50 - 1/100.

Target

  • Relevance

    Transporter that mediates epithelial resorption of neutral amino acids across the apical membrane of epithelial cells in the kidney and intestine. It appears that leucine is the preferred substrate, but all large neutral non-aromatic L-amino acids bind to this transporter. Uptake of leucine is sodium-dependent. In contrast to other members of the neurotransmitter transporter family, does not appear to be chloride-dependent

  • Database links

    • Alternative names

      • B(0)AT1 antibody
      • Sodium dependent neutral amino acid transporter B(0) antibody
      • Solute carrier family 6 member 19 antibody
      • System B(0) neutral amino acid transporter antibody

    Images

    • Western blot - Anti-SLC6A19 antibody [EPR14154(B)] (ab180516)
      Western blot - Anti-SLC6A19 antibody [EPR14154(B)] (ab180516)
      All lanes : Anti-SLC6A19 antibody [EPR14154(B)] (ab180516) at 1/1000 dilution (Purified)

      Lane 1 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
      Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 3 : Human kidney lysate
      Lane 4 : Mouse kidney lysate
      Lane 5 : Mouse brain lysate
      Lane 6 : Rat brain lysate
      Lane 7 : Mouse heart lysate
      Lane 8 : Rat heart lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 71 kDa
      Observed band size: 75 kDa
      why is the actual band size different from the predicted?



      The molecular weight observed is consistent with what has been described in the literature (PMID: 25884559).

      Blocking Buffer and concentration: 5% NFDM/TBST

    • Immunocytochemistry - Anti-SLC6A19 antibody [EPR14154(B)] (ab180516)
      Immunocytochemistry - Anti-SLC6A19 antibody [EPR14154(B)] (ab180516)

      Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling SLC6A19 with Purified ab180516 at 1:50 dilution (2.62 ?g/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A19 antibody [EPR14154(B)] (ab180516)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A19 antibody [EPR14154(B)] (ab180516)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling SLC6A19 with Purified ab180516 at 1:100 dilution (1.31 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A19 antibody [EPR14154(B)] (ab180516)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A19 antibody [EPR14154(B)] (ab180516)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse colon tissue sections labeling SLC6A19 with Purified ab180516 at 1:100 dilution (1.31 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A19 antibody [EPR14154(B)] (ab180516)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A19 antibody [EPR14154(B)] (ab180516)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling SLC6A19 with Purified ab180516 at 1:100 dilution (1.31 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • ELISA - Anti-SLC6A19 antibody [EPR14154(B)] (ab180516)
      ELISA - Anti-SLC6A19 antibody [EPR14154(B)] (ab180516)

      ELISA analysis of Human SLC6A19 recombinant protein at 500 ng/mL with ab180516. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

    • Anti-SLC6A19 antibody [EPR14154(B)] (ab180516)
      Anti-SLC6A19 antibody [EPR14154(B)] (ab180516)

    References (2)

    Publishing research using ab180516? Please let us know so that we can cite the reference in this datasheet.

    ab180516 has been referenced in 2 publications.

    • Yang X  et al. Effects of chronic stress on intestinal amino acid pathways. Physiol Behav 204:199-209 (2019). PubMed: 30831184
    • Zhao Y  et al. SLC6A1-miR133a-CDX2 loop regulates SK-OV-3 ovarian cancer cell proliferation, migration and invasion. Oncol Lett 16:4977-4983 (2018). PubMed: 30250563

    Customer reviews and Q&As

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    Immunohistochemistry (Frozen sections) abreview for Anti-SLC6A19 antibody [EPR14154(B)]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Cholangiocytes)
    Permeabilization
    Yes - 0.1% Triton X
    Specification
    Cholangiocytes
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

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    Verified customer

    Submitted Sep 15 2020

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