Recombinant
RabMAb

Recombinant Anti-SLC6A19 antibody [EPR14154(B)] (ab180516)

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Overview

  • Product name

    Anti-SLC6A19 antibody [EPR14154(B)]
    See all SLC6A19 primary antibodies

  • Description

    Rabbit monoclonal [EPR14154(B)] to SLC6A19

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human SLC6A19 aa 350-450 (internal sequence). The exact sequence is proprietary.
    Database link: Q695T7

  • Positive control

    • PC3 Caco2, HeLa and 293 cell lysates, HeLa cells and paraffin embedded Human kidney tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR14154(B)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab180516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 71 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/500.

Target

  • Relevance

    Transporter that mediates epithelial resorption of neutral amino acids across the apical membrane of epithelial cells in the kidney and intestine. It appears that leucine is the preferred substrate, but all large neutral non-aromatic L-amino acids bind to this transporter. Uptake of leucine is sodium-dependent. In contrast to other members of the neurotransmitter transporter family, does not appear to be chloride-dependent

  • Database links

    • Alternative names

      • B(0)AT1 antibody
      • Sodium dependent neutral amino acid transporter B(0) antibody
      • Solute carrier family 6 member 19 antibody
      • System B(0) neutral amino acid transporter antibody

    Images

    • Western blot - Anti-SLC6A19 antibody [EPR14154(B)] (ab180516)
      Western blot - Anti-SLC6A19 antibody [EPR14154(B)] (ab180516)
      All lanes : Anti-SLC6A19 antibody [EPR14154(B)] (ab180516) at 1/10000 dilution

      Lane 1 : PC3 cell lysate
      Lane 2 : Caco2 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : 293 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit IgG (H+L) Peroxidase conjugated secondary antibody at 1/1000 dilution

      Predicted band size: 71 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A19 antibody [EPR14154(B)] (ab180516)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A19 antibody [EPR14154(B)] (ab180516)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SLC6A19 with ab180516 at 1/100 dilution. Anti-rabbbit HRP secondary antibody was used and cells counterstained with Hematoxylin.  

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-SLC6A19 antibody [EPR14154(B)] (ab180516)
      Immunocytochemistry/ Immunofluorescence - Anti-SLC6A19 antibody [EPR14154(B)] (ab180516)

      Immunofluorescent analysis of HeLa cells labeling SLC6A19 with ab180516 at 1/500 dilution. Cells were fixed with 20% Acetone and 

      Goat anti rabbit IgG was used at 1/250 diluton as secondary antibody. Cells were counter-stained with DAPI.

    References

    This product has been referenced in:

    • Zhao Y  et al. SLC6A1-miR133a-CDX2 loop regulates SK-OV-3 ovarian cancer cell proliferation, migration and invasion. Oncol Lett 16:4977-4983 (2018). Read more (PubMed: 30250563) »
    See 1 Publication for this product

    Publishing research using ab180516? Please let us know so that we can cite the reference in this datasheet.

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