Product nameAnti-SLC6A19 antibody [EPR14154(B)]
See all SLC6A19 primary antibodies
DescriptionRabbit monoclonal [EPR14154(B)] to SLC6A19
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human SLC6A19 aa 350-450 (internal sequence). The exact sequence is proprietary.
Database link: Q695T7
- PC3 Caco2, HeLa and 293 cell lysates, HeLa cells and paraffin embedded Human kidney tissue.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab180516 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 71 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
RelevanceTransporter that mediates epithelial resorption of neutral amino acids across the apical membrane of epithelial cells in the kidney and intestine. It appears that leucine is the preferred substrate, but all large neutral non-aromatic L-amino acids bind to this transporter. Uptake of leucine is sodium-dependent. In contrast to other members of the neurotransmitter transporter family, does not appear to be chloride-dependent
- B(0)AT1 antibody
- Sodium dependent neutral amino acid transporter B(0) antibody
- Solute carrier family 6 member 19 antibody
- System B(0) neutral amino acid transporter antibody
All lanes : Anti-SLC6A19 antibody [EPR14154(B)] (ab180516) at 1/10000 dilution
Lane 1 : PC3 cell lysate
Lane 2 : Caco2 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat anti-rabbit IgG (H+L) Peroxidase conjugated secondary antibody at 1/1000 dilution
Predicted band size: 71 kDa
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SLC6A19 with ab180516 at 1/100 dilution. Anti-rabbbit HRP secondary antibody was used and cells counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunofluorescent analysis of HeLa cells labeling SLC6A19 with ab180516 at 1/500 dilution. Cells were fixed with 20% Acetone and
Goat anti rabbit IgG was used at 1/250 diluton as secondary antibody. Cells were counter-stained with DAPI.