Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14154(B)] to SLC6A19 - BSA and Azide free
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Product nameAnti-SLC6A19 antibody [EPR14154(B)] - BSA and Azide free
See all SLC6A19 primary antibodies
DescriptionRabbit monoclonal [EPR14154(B)] to SLC6A19 - BSA and Azide free
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human SLC6A19 aa 350-450 (internal sequence). The exact sequence is proprietary.
Database link: Q695T7
- WB: PC-3 Caco-2, HeLa and HEK-293 cell lysates. ICC: HeLa cells. IHC-P: Human kidney tissue.
Ab235386 is the carrier-free version of ab180516. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab235386 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab235386 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 71 kDa.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|ICC/IF||Use at an assay dependent concentration.|
RelevanceTransporter that mediates epithelial resorption of neutral amino acids across the apical membrane of epithelial cells in the kidney and intestine. It appears that leucine is the preferred substrate, but all large neutral non-aromatic L-amino acids bind to this transporter. Uptake of leucine is sodium-dependent. In contrast to other members of the neurotransmitter transporter family, does not appear to be chloride-dependent
- B(0)AT1 antibody
- Sodium dependent neutral amino acid transporter B(0) antibody
- Solute carrier family 6 member 19 antibody
- System B(0) neutral amino acid transporter antibody
This IHC data was generated using the same anti-SLCA19 antibody clone, EPR14154(B), in a different buffer formulation (cat# ab180516).
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling SLC6A19 with ab180516 at 1/100 dilution. Anti-rabbbit HRP secondary antibody was used and cells counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235386 has not yet been referenced specifically in any publications.