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    slc6a7-antibody-ab122024.pdf

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Neuroscience Neurotransmission Receptors / Channels Sodium Channels
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Anti-SLC6A7 antibody (ab122024)

  • Datasheet
  • SDS
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Immunocytochemistry/ Immunofluorescence - Anti-SLC6A7 antibody (ab122024)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A7 antibody (ab122024)

Key features and details

  • Rabbit polyclonal to SLC6A7
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-SLC6A7 antibody
  • Description

    Rabbit polyclonal to SLC6A7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    antigen sequence: SLTSDLPWEH CGNWWNTELC LEHRVSKDGN GALPLNLTCT VSPSEEYWSR YVLHIQGSQG IGSPG, corresponding to amino acids 147-211 of Human SLC6A7

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human colon tissue
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Sodium Channels
    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • Channels

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab122024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
ICC/IF
Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Terminates the action of proline by its high affinity sodium-dependent reuptake into presynaptic terminals.
  • Tissue specificity

    Brain.
  • Sequence similarities

    Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A7 subfamily.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession Q99884 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6534 Human
    • Omim: 606205 Human
    • SwissProt: Q99884 Human
    • Unigene: 241597 Human
    • Alternative names

      • solute carrier family 6 (neurotransmitter transporter, L-proline), member 7 antibody
      • brain-specific L-proline transporter antibody
      • L-proline transporter, brain-specific antibody
      • PROT antibody
      • SC6A7_HUMAN antibody
      • Slc6a7 antibody
      • Sodium-dependent proline transporter antibody
      • solute carrier family 6 (neurotransmitter transporter), member 7 antibody
      • Solute carrier family 6 member 7 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-SLC6A7 antibody (ab122024)
      Immunocytochemistry/ Immunofluorescence - Anti-SLC6A7 antibody (ab122024)
      Immunofluorescent staining of Human cell line U-251MG shows positivity in cytoplasm. Recommended concentration of ab122024 1-4 µg/ml. Cells treated with PFA/Triton X-100.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A7 antibody (ab122024)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A7 antibody (ab122024)
      ab122024, at a 1/20 dilution, staining SLC6A7 in paraffin-embedded Human colon tissue, by Immunohistochemistry.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab122024? Please let us know so that we can cite the reference in this datasheet.

    ab122024 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Immunocytochemistry abreview for Anti-SLC6A7 antibody

    Excellent
    Abreviews
    Abreviews
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (CHO)
    Specification
    CHO
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.3% triton
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 18 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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