Anti-SLC6A7 antibody (ab122024)

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Overview

  • Product name

    Anti-SLC6A7 antibody

  • Description

    Rabbit polyclonal to SLC6A7

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    antigen sequence: SLTSDLPWEH CGNWWNTELC LEHRVSKDGN GALPLNLTCT VSPSEEYWSR YVLHIQGSQG IGSPG, corresponding to amino acids 147-211 of Human SLC6A7

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human colon tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab122024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Terminates the action of proline by its high affinity sodium-dependent reuptake into presynaptic terminals.

  • Tissue specificity

    Brain.

  • Sequence similarities

    Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A7 subfamily.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • solute carrier family 6 (neurotransmitter transporter, L-proline), member 7 antibody
      • brain-specific L-proline transporter antibody
      • L-proline transporter, brain-specific antibody
      • PROT antibody
      • SC6A7_HUMAN antibody
      • Slc6a7 antibody
      • Sodium-dependent proline transporter antibody
      • solute carrier family 6 (neurotransmitter transporter), member 7 antibody
      • Solute carrier family 6 member 7 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-SLC6A7 antibody (ab122024)
      Immunocytochemistry/ Immunofluorescence - Anti-SLC6A7 antibody (ab122024)
      Immunofluorescent staining of Human cell line U-251MG shows positivity in cytoplasm. Recommended concentration of ab122024 1-4 µg/ml. Cells treated with PFA/Triton X-100.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A7 antibody (ab122024)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A7 antibody (ab122024)
      ab122024, at a 1/20 dilution, staining SLC6A7 in paraffin-embedded Human colon tissue, by Immunohistochemistry.

    References

    ab122024 has not yet been referenced specifically in any publications.

    Publishing research using ab122024? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Immunocytochemistry abreview for Anti-SLC6A7 antibody

     Excellent
    Abreviews
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (CHO)
    Specification
    CHO
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.3% triton
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 18 2013

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