Anti-SLC6A7 antibody (ab122024)
Key features and details
- Rabbit polyclonal to SLC6A7
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-SLC6A7 antibody -
Description
Rabbit polyclonal to SLC6A7 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: SLTSDLPWEH CGNWWNTELC LEHRVSKDGN GALPLNLTCT VSPSEEYWSR YVLHIQGSQG IGSPG, corresponding to amino acids 147-211 of Human SLC6A7
-
Positive control
- Human colon tissue
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122024 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment |
|
| IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
ICC/IF
Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
|
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Terminates the action of proline by its high affinity sodium-dependent reuptake into presynaptic terminals. -
Tissue specificity
Brain. -
Sequence similarities
Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A7 subfamily. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 6534 Human
- Omim: 606205 Human
- SwissProt: Q99884 Human
- Unigene: 241597 Human
-
Alternative names
- solute carrier family 6 (neurotransmitter transporter, L-proline), member 7 antibody
- brain-specific L-proline transporter antibody
- L-proline transporter, brain-specific antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-SLC6A7 antibody (ab122024)Immunofluorescent staining of Human cell line U-251MG shows positivity in cytoplasm. Recommended concentration of ab122024 1-4 µg/ml. Cells treated with PFA/Triton X-100.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A7 antibody (ab122024)ab122024, at a 1/20 dilution, staining SLC6A7 in paraffin-embedded Human colon tissue, by Immunohistochemistry.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab122024 has not yet been referenced specifically in any publications.