Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab18493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    Sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Involved in cellular amino acid uptake. Acts as an amino acid exchanger. Involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Plays a role in neuronal cell proliferation (neurogenesis) in brain. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. May play an important role in high-grade gliomas. Mediates blood-to-retina L-leucine transport across the inner blood-retinal barrier which in turn may play a key role in maintaining large neutral amino acids as well as neurotransmitters in the neural retina. Acts as the major transporter of tyrosine in fibroblasts.
  • Tissue specificity
    Expressed abundantly in adult lung, liver, brain, skeletal muscle, placenta, bone marrow, testis, resting lymphocytes and monocytes, and in fetal liver. Weaker expression in thymus, cornea, retina, peripheral leukocytes, spleen, kidney, colon and lymph node. During gestation, expression in the placenta was significantly stronger at full-term than at the mid-trimester stage. Also expressed in all human tumor cell lines tested and in the astrocytic process of primary astrocytic gliomas. Expressed in retinal endothelial cells and in the intestinal epithelial cell line Caco-2.
  • Sequence similarities
    Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.
  • Cellular localization
    Cytoplasm > cytosol. Apical cell membrane. Located to the plasma membrane by SLC3A2/4F2hc. Localized to the apical membrane of placental syncytiophoblastic cells. Expressed in both luminal and abluminal membranes of brain capillary endothelial cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4F2 LC antibody
    • 4F2 light chain antibody
    • 4F2LC antibody
    • CD98 antibody
    • CD98 light chain antibody
    • CD98LC antibody
    • D16S469E antibody
    • DC49 antibody
    • E16 antibody
    • hLAT1 antibody
    • Integral membrane protein E16 antibody
    • L type amino acid transporter antibody
    • L type amino acid transporter 1 antibody
    • L-type amino acid transporter 1 antibody
    • Large neutral amino acids transporter 1 antibody
    • Large neutral amino acids transporter antibody
    • Large neutral amino acids transporter small subunit 1 antibody
    • LAT1 antibody
    • LAT1_HUMAN antibody
    • Membrane protein E16 antibody
    • MPE16 antibody
    • Slc7a5 antibody
    • Sodium independent neutral amino acid transporter antibody
    • Sodium independent neutral amino acid transporter LAT1 antibody
    • Solute carrier family 7 (cationic amino acid transporter y+ system) member 5 antibody
    • Solute carrier family 7 member 5 antibody
    • TA1 antibody
    • Tumor associated protein 1 antibody
    • y+ system cationic amino acid transporter antibody
    see all

Images

  • ICC/IF image of ab18493 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18493, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Tattoli I  et al. The bacterial and cellular determinants controlling the recruitment of mTOR to the Salmonella-containing vacuole. Biol Open 1:1215-25 (2012). ICC/IF ; Human . Read more (PubMed: 23259056) »
See 1 Publication for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thank you for your inquiry. I have to confirm that it is very un-likely that ab18493 will cross react with mouse Slc7a5 Lat1 because the homology between the immunogen and the same sequence in mouse is below 75%. This antibody is also not yet tested and guaranteed for WB. I am sorry I do not have a positive answer on this occasion and hope this information is nevertheless helpful. Please do not hesitate to contact me again with any further questions.

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Question
Answer

Thank you for your enquiry. This antibody was made the a sequence near the C terminus of the protein. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your enquiry. My recommendation of a Saponin permeabilization was due to the fact that this antibody is targeting a membraneous protein and therefore triton is likely to over permeabilise the membrane, in doing so potentially remove the protein that the the antibody is directed against. The response that I mailed to Yingying Wang was as follows; "Thank you for getting back to me. Unfortunately we do not have a specific protocol for this antibody. However, for the saponin treatment of formalin-fixated or paraffin-embedded cells I would like to recommend giving your sections a 30 minute pre-treatment in 0.05% saponin in distilled water at room temperature. Wash at least three times in PBS. However, given that you have been using paraformaldehyde I would recommend replacing your triton with 0.5% saponin in the following protocol; https://www.abcam.com/assets/pdf/protocols/Immunocytochemistry%20(ICC)%20protocol.pdf I hope this information helps, please do not hesitate to contact us if you need any more advice or information".

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Answer

Thank you for getting back to me. Unfortunately we do not have a specific protocol for this antibody. However, for the saponin treatment of formalin-fixated or paraffin-embedded cells I would like to recommend giving your sections a 30 minute pre-treatment in 0.05% saponin in distilled water at room temperature. Wash at least three times in PBS. However, given that you have been using paraformaldehyde I would recommend replacing your triton with 0.5% saponin in the following protocol; https://www.abcam.com/assets/pdf/protocols/Immunocytochemistry%20(ICC)%20protocol.pdf I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting me regarding the problems that you have experienced using this Ab. I am sorry to hear that your customer has been having difficulties using this antibody. I have read their technical questionaire and I have a few comments. At this stage I would like to suggest applying the antibody at a dilution of 1/100 as they have been in conjunction with using saponin as a permeabilization reagent (5-10ug/ml) rather than Triton X-100. Triton-X 100 will destroy cellular membranes. Since the target is indeed membraneous this may affect the available antigen. Saponin is a detergent that permeabilizes the cell membrane of eukaryotic cell membranes by interacting with cholesterol and is more gentle than Triton-X. Where membranes are not over treated with saponin they have the ability to reform. Please do not hesitate to get back in touch with me should you still experience problems.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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