Overview

  • Product name

  • Description

    Rabbit polyclonal to SLC7A5/LAT1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human SLC7A5/LAT1 (N terminal).
    Database link: Q01650
    (Peptide available as ab192836)

  • Positive control

    • Human lung lysate; human lung tissue.

Properties

  • Form

    Lyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab85226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/100 - 1/1000. Predicted molecular weight: 55 kDa.Can be blocked with SLC7A5/LAT1 peptide (ab192836).
IHC-P 1/50 - 1/250.

Target

  • Function

    Sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Involved in cellular amino acid uptake. Acts as an amino acid exchanger. Involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Plays a role in neuronal cell proliferation (neurogenesis) in brain. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. May play an important role in high-grade gliomas. Mediates blood-to-retina L-leucine transport across the inner blood-retinal barrier which in turn may play a key role in maintaining large neutral amino acids as well as neurotransmitters in the neural retina. Acts as the major transporter of tyrosine in fibroblasts.
  • Tissue specificity

    Expressed abundantly in adult lung, liver, brain, skeletal muscle, placenta, bone marrow, testis, resting lymphocytes and monocytes, and in fetal liver. Weaker expression in thymus, cornea, retina, peripheral leukocytes, spleen, kidney, colon and lymph node. During gestation, expression in the placenta was significantly stronger at full-term than at the mid-trimester stage. Also expressed in all human tumor cell lines tested and in the astrocytic process of primary astrocytic gliomas. Expressed in retinal endothelial cells and in the intestinal epithelial cell line Caco-2.
  • Sequence similarities

    Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.
  • Cellular localization

    Cytoplasm > cytosol. Apical cell membrane. Located to the plasma membrane by SLC3A2/4F2hc. Localized to the apical membrane of placental syncytiophoblastic cells. Expressed in both luminal and abluminal membranes of brain capillary endothelial cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • 4F2 LC antibody
    • 4F2 light chain antibody
    • 4F2LC antibody
    • CD98 antibody
    • CD98 light chain antibody
    • CD98LC antibody
    • D16S469E antibody
    • DC49 antibody
    • E16 antibody
    • hLAT1 antibody
    • Integral membrane protein E16 antibody
    • L type amino acid transporter antibody
    • L type amino acid transporter 1 antibody
    • L-type amino acid transporter 1 antibody
    • Large neutral amino acids transporter 1 antibody
    • Large neutral amino acids transporter antibody
    • Large neutral amino acids transporter small subunit 1 antibody
    • LAT1 antibody
    • LAT1_HUMAN antibody
    • Membrane protein E16 antibody
    • MPE16 antibody
    • Slc7a5 antibody
    • Sodium independent neutral amino acid transporter antibody
    • Sodium independent neutral amino acid transporter LAT1 antibody
    • Solute carrier family 7 (cationic amino acid transporter y+ system) member 5 antibody
    • Solute carrier family 7 member 5 antibody
    • TA1 antibody
    • Tumor associated protein 1 antibody
    • y+ system cationic amino acid transporter antibody
    see all

Images

  • Anti-SLC7A5/LAT1 antibody (ab85226) + Human SW480 (colorectal adenocarcinoma) cell line at 1/500 dilution

    Predicted band size: 55 kDa

  • Anti-SLC7A5/LAT1 antibody (ab85226) at 1/200 dilution + human lung lysate

    Predicted band size: 55 kDa
    Observed band size: 57 kDa
    why is the actual band size different from the predicted?

  • ICC/IF image of ab85226 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85226, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab85226, at 1/100 dilution, staining SLC7A5/LAT1 in formalin-fixed, paraffin-embedded human lung by Immunohistochemistry.

References

This product has been referenced in:

  • Cairns J  et al. Differential roles of ERRFI1 in EGFR and AKT pathway regulation affect cancer proliferation. EMBO Rep 19:N/A (2018). Read more (PubMed: 29335246) »
  • Barthelemy C  et al. FTY720-induced endocytosis of yeast and human amino acid transporters is preceded by reduction of their inherent activity and TORC1 inhibition. Sci Rep 7:13816 (2017). WB ; Human . Read more (PubMed: 29062000) »
See all 9 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
80 µg
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Miss. Dora Tarlungeanu

Verified customer

Submitted Dec 17 2015

Answer

Thank you for contacting us.

Yes, both ab85226 and ab134121 detect the same SLC7A5 (LAT1) protein. http://www.uniprot.org/uniprot/Q01650

We would guarantee ab134121 to work in WB, IP, and IHC-P with human samples. We know that it does not work well with Flow Cyt, ICC/IF and rat or mouse samples.

Cat # ab89545 is an EEA1 antibody no longer available on our catalogue. You must mean ab29545, the HeLa cell lysate. Yes, that would be a good positive control for use with ab134121.

In WB with ab134141, we recommend a working dilution of 1/1000 - 1/10000. The amount that you will need will depend on the amount present in your samples. The image generated on our website was done with a 1/1000 dilution for example.

A suitable secondary would be Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) to be used with ECL detection, like our ab133406.

https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-HL-HRP-ab97051.html

https://www.abcam.com/optiblot-ecl-detect-kit-23pg-187ng-ab133406.html

You can find our full range of Optiblot WB reagents here: https://www.abcam.com/index.html?pageconfig=resource&rid=14709&source=pagetrap&viapagetrap=optiblot

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Question
Answer

Thanks for your inquiry. From all the information available, there is no preferred antigen retrieval step for ab85226. I have attached the standard antigen retrieval step for IHC as part of the IHC-P protocol. Please refer to the antigen retrieval part. I hope this was helpful. Please feel free to contact me if you require further assistance.

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