Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab136820 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab136820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 103 kDa.
A 1 hour primary incubation is recommended for this product. Preliminary experiments gave an approx 28kDa band in Mouse Brain lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 102kDa according to NP_001161392.1. The 28kDa band was successfully blocked by incubation with the immunizing peptide. We would welcome your feedback if you have tested this antibody in more suitable positive controls.
RelevanceSLC8A3 is a member of the sodium/calcium exchanger integral membrane protein family. Three mammalian isoforms in family 8 have been identified. Na+/Ca2+ exchange proteins are involved in maintaining Ca2+ homeostasis in a wide variety of cell types. The protein is regulated by intracellular calcium ions and is found in both the plasma membrane and intracellular organellar membranes, where exchange of Na+ for Ca2+ occurs in an electrogenic manner.
Cellular localizationMembrane; Multi-pass membrane protein.
- AW742262 antibody
- MGC90626 antibody
- Na+/Ca2+ exchange protein 3 antibody
ab136820 has not yet been referenced specifically in any publications.