Overview

  • Product name

    Anti-SLC9A9 antibody [EP8725]
  • Description

    Rabbit monoclonal [EP8725] to SLC9A9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SLC9A9. The exact sequence is proprietary.

  • Positive control

    • A549, Caco-2, HeLa and HepG2 whole cell lysate (ab7900). Human kidney tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP8725
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab167157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 73 kDa.
IHC-P 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      May act in electroneutral exchange of protons for Na(+) across membranes. Involved in the effusion of Golgi luminal H(+) in exchange for cytosolic cations. Involved in organelle ion homeostasis by contributing to the maintainance of the unique acidic pH values of the Golgi and post-Golgi compartments in the cell.
    • Tissue specificity

      Ubiquitously expressed in all tissues tested. Expressed at highest levels in heart and skeletal muscle, followed by placenta, kidney, and liver. Expressed in the brain, in the medulla and spinal cord.
    • Involvement in disease

      Note=A chromosomal aberration involving SLC9A9 has been found in a family with early-onset behavioral/developmental disorder with features of attention deficit-hyperactivity disorder and intellectual disability. Inversion inv(3)(p14:q21). The inversion disrupts DOCK3 and SLC9A9.
      Defects in SLC9A9 are a cause of susceptibility to autism type 16 (AUTS16) [MIM:613410]. Autism is a complex multifactorial, pervasive developmental disorder characterized by impairments in reciprocal social interaction and communication, restricted and stereotyped patterns of interests and activities, and the presence of developmental abnormalities by 3 years of age. Most individuals with autism also manifest moderate mental retardation. AUTS16 can be associated with epilepsy.
    • Sequence similarities

      Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.
    • Cellular localization

      Late endosome membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • 5730527A11Rik antibody
      • 9930105B05 antibody
      • AI854429 antibody
      • FLJ35613 antibody
      • Na(+)/H(+) exchanger 9 antibody
      • Nbla00118 antibody
      • NHE 9 antibody
      • NHE-9 antibody
      • NHE9 antibody
      • Putative protein product of Nbla00118 antibody
      • SL9A9_HUMAN antibody
      • Slc9a9 antibody
      • Sodium/hydrogen exchanger 9 antibody
      • Sodium/proton exchanger NHE9 antibody
      • Solute carrier family 9 (sodium/hydrogen exchanger) isoform 9 antibody
      • Solute carrier family 9 (sodium/hydrogen exchanger) member 9 antibody
      • Solute carrier family 9 member 9 antibody
      see all

    Images

    • All lanes : Anti-SLC9A9 antibody [EP8725] (ab167157) at 1/1000 dilution

      Lane 1 : A549 cell lysate
      Lane 2 : Caco 2 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 73 kDa

    • Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling SLC9A9 with ab167157 at a 1/100 dilution.

    References

    This product has been referenced in:

    • Lucien F  et al. Hypoxia-induced mobilization of NHE6 to the plasma membrane triggers endosome hyperacidification and chemoresistance. Nat Commun 8:15884 (2017). Read more (PubMed: 28635961) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab167157.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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