Anti-Slco1a1/Oatp1a1 antibody (ab203036)

Overview

  • Product name

    Anti-Slco1a1/Oatp1a1 antibody
  • Description

    Rabbit polyclonal to Slco1a1/Oatp1a1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Rat Slco1a1/Oatp1a1 aa 1-50 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    MEETEKKIAT QEGRLFSKMK VFLLSLTCAC LTKSLSGVYM NSMLTQIERQ


    Database link: P46720

  • Positive control

    • Mouse liver lysate. Mouse kidney tisse.
  • General notes

     This product was previously labelled as Slco1a1

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab203036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000. Predicted molecular weight: 74 kDa.
IHC-P 1/50 - 1/500.

Target

  • Function

    Mediates the Na(+)-independent transport of organic anions such as bromosulfobromophthalein (BSP) and conjugated (taurocholate) and unconjugated (cholate) bile acids.
  • Tissue specificity

    Highly expressed in liver and kidney, and at lower levels in brain, lung, skeletal muscle and proximal colon.
  • Sequence similarities

    Belongs to the organo anion transporter (TC 2.A.60) family.
    Contains 1 Kazal-like domain.
  • Post-translational
    modifications

    Glycosylated.
  • Cellular localization

    Basolateral cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • OATP-1 antibody
    • Organic anion-transporting polypeptide 1 antibody
    • Slco1a1 antibody
    • SO1A1_RAT antibody
    • Sodium-independent organic anion transporter 1 antibody
    • Solute carrier family 21 member 1 antibody
    • Solute carrier organic anion transporter family member 1A1 antibody
    see all

Images

  • All lanes : Anti-Slco1a1/Oatp1a1 antibody (ab203036) at 1/200 dilution

    Lane 1 : Mouse liver lysate
    Lane 2 : Rat brain lysate

    Secondary
    All lanes : HRP conjugated secondary at 1/3000 dilution

    Predicted band size: 74 kDa
    Additional bands at: 56 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemical analysis of formalin-fixed and paraffin embedded mouse kidney tissue sections labeling Slco1a1/Oatp1a1 with ab203036 at 1/200 followed by conjugation to the secondary antibody and DAB staining.

References

ab203036 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (Ran on Wes from Protein Simple)
Loading amount
4 µg
Specification
Liver
Blocking step
Milk as blocking agent for 5 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Oct 25 2017

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