Anti-SLCO1B3/OATP1B3 antibody (ab222900)
Key features and details
- Rabbit polyclonal to SLCO1B3/OATP1B3
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SLCO1B3/OATP1B3 antibody
See all SLCO1B3/OATP1B3 primary antibodies -
Description
Rabbit polyclonal to SLCO1B3/OATP1B3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human SLCO1B3/OATP1B3 aa 600-700.
Database link: Q9NPD5 -
Positive control
- IHC-P: Human liver and liver cancer tissue. ICC/IF: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab222900 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/20 - 1/200.
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ICC/IF |
1/50 - 1/200.
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Notes |
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IHC-P
1/20 - 1/200. |
ICC/IF
1/50 - 1/200. |
Target
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Function
Mediates the Na(+)-independent uptake of organic anions such as 17-beta-glucuronosyl estradiol, taurocholate, triiodothyronine (T3), leukotriene C4, dehydroepiandrosterone sulfate (DHEAS), methotrexate and sulfobromophthalein (BSP). Involved in the clearance of bile acids and organic anions from the liver. -
Tissue specificity
Highly expressed in liver, in particular at the basolateral membrane of hepatocytes near the central vein. Not detected in other tissues. Highly expressed in some cancer cell lines derived from colon, pancreas, liver and gall bladder. -
Involvement in disease
Hyperbilirubinemia, Rotor type -
Sequence similarities
Belongs to the organo anion transporter (TC 2.A.60) family.
Contains 1 Kazal-like domain. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Basolateral cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 28234 Human
- Omim: 605495 Human
- SwissProt: Q9NPD5 Human
- Unigene: 504966 Human
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Alternative names
- HBLRR antibody
- Liver-specific organic anion transporter 2 antibody
- LST-2 antibody
see all
Images
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HepG2 (human liver hepatocellular carcinoma cell line) cells stained for SLCO1B3/OATP1B3 (green) using ab222900 (1/100 dilution) in ICC/IF. Secondary: Alexa Fluor 488®-conjugated Goat Anti-Rabbit IgG (H+L).
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Paraffin embedded human liver cancer tissue stained for SLCO1B3/OATP1B3 with ab222900 (1/100 dilution) in immunohistochemical analysis.
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Paraffin embedded human liver tissue stained for SLCO1B3/OATP1B3 with ab222900 (1/100 dilution) in immunohistochemical analysis.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab222900 has been referenced in 1 publication.
- Chen S et al. Low expression of organic anion-transporting polypeptide 1B3 predicts a poor prognosis in hepatocellular carcinoma. World J Surg Oncol 18:127 (2020). PubMed: 32534581