The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 77 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
SLCO2B1 (solute carrier organic anion transporter family member 2B1), or Organic anion transporter B (OATP-B) mediates sodium-independent transport of organic anions. Unlike other related proteins, SLCO2B1 is expressed in many tissues, including liver, placenta, brain, heart and intestine. SLCO2B1 plays a role in the uptake of steroids and a number of drugs such as statins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling SLCO2B1 with ab83532 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the membrane of alveolar type I cells. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - SLCO2B1. Right - Merge.
Western blot - Anti-SLCO2B1 antibody (ab83532)
Anti-SLCO2B1 antibody (ab83532) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 77 kDa Observed band size: 77 kDa
Riha J et al. Resveratrol and its major sulfated conjugates are substrates of organic anion transporting polypeptides (OATPs): impact on growth of ZR-75-1 breast cancer cells. Mol Nutr Food Res58:1830-42 (2014).
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