Recombinant
RabMAb

Recombinant Anti-SLCO2B1/OATP2B1 antibody [EPR22212] (ab222094)

Overview

  • Product name

    Anti-SLCO2B1/OATP2B1 antibody [EPR22212]
    See all SLCO2B1/OATP2B1 primary antibodies
  • Description

    Rabbit monoclonal [EPR22212] to SLCO2B1/OATP2B1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SLCO2B1/OATP2B1 aa 650 to the C-terminus. The exact sequence is proprietary.
    Database link: O94956

  • Positive control

    • IHC-P: Human liver and placenta tissue. Flow Cyt: Huh7 cells.
  • General notes

     This product was previously labelled as SLCO2B1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab222094 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt 1/50.

Target

  • Relevance

    SLCO2B1 (solute carrier organic anion transporter family member 2B1), or Organic anion transporter B (OATP-B) mediates sodium-independent transport of organic anions. Unlike other related proteins, SLCO2B1 is expressed in many tissues, including liver, placenta, brain, heart and intestine. SLCO2B1 plays a role in the uptake of steroids and a number of drugs such as statins.
  • Cellular localization

    Cell Membrane
  • Database links

  • Alternative names

    • Moat 1 antibody
    • Moat1 antibody
    • Multispecific organic anion transporter 1 antibody
    • Multispecific organic anion transporter1 antibody
    • OATP 2B1 antibody
    • OATP B antibody
    • OATP RP2 antibody
    • OATP2 B1 antibody
    • OATP2B1 antibody
    • OATPB antibody
    • OATPRP 2 antibody
    • OATPRP2 antibody
    • Organic anion transporter B antibody
    • Organic anion transporter polypeptide related protein 2 antibody
    • Organic anion transporter polypeptide related protein2 antibody
    • SLC 21A9 antibody
    • SLC2 1A9 antibody
    • SLC21 A9 antibody
    • SLC21A9 antibody
    • SLCO 2B1 antibody
    • SLCO2 B1 antibody
    • SO 2B1 antibody
    • SO2 B1 antibody
    • SO2B1 antibody
    • Solute carrier family 21 (organic anion transporter), member 9 antibody
    • Solute carrier family 21 member 9 antibody
    • Solute carrier organic anion transporter family member 2B1 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling SLCO2B1/OATP2B1 with ab222094 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Basolateral membranous staining on human placenta (PMID: 17020956; PMID: 20159975) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

  • Flow cytometric analysis of Huh7 (human hepatocellular carcinoma epithelial cell line) cells labeling SLCO2B1/OATP2B1 with ab222094 at 1/50 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue labeling SLCO2B1/OATP2B1 with ab222094 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous staining on human liver (PMID: 17178262; PMID: 11159893; PMID: 23987090) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

References

ab222094 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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