Product nameAnti-Sld2 antibody
Species reactivityReacts with: Saccharomyces cerevisiae
Synthetic peptide corresponding to Saccharomyces cerevisiae Sld2 aa 200-300 conjugated to keyhole limpet haemocyanin.
Database link: P34252
- This antibody gave a positive signal in both Exponentially Growing WT Yeast and W303 S.cerevisiae lysates
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
|WB||Use at an assay dependent concentration. Predicted molecular weight: 52 kDa.|
- Information by UniProt
- DNA replication and checkpoint protein 1 antibody
- DNA replication regulator SLD2 antibody
- SLD2 antibody
- SLD2_YEAST antibody
All lanes : Anti-Sld2 antibody (ab139172) at 1 µg/ml
Lane 1 : Exponentially Growing WT Yeast Whole Cell Lysate
Lane 2 : W303 Yeast Lysate Whole Cell Lysate - Negative Control
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 61 kDa why is the actual band size different from the predicted?
Additional bands at: 29 kDa (possible non-specific binding), 42 kDa (possible non-specific binding), 67 kDa (possible non-specific binding)
Exposure time: 30 seconds
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab139172 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab139172 has not yet been referenced specifically in any publications.