Overview

  • Product name

    Anti-Sld2 antibody
  • Description

    Rabbit polyclonal to Sld2

    This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

  • Host species

    Rabbit
  • Species reactivity

    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide corresponding to Saccharomyces cerevisiae Sld2 aa 200-300 conjugated to keyhole limpet haemocyanin.
    Database link: P34252

  • Positive control

    • This antibody gave a positive signal in both Exponentially Growing WT Yeast and W303 S.cerevisiae lysates

Properties

Applications

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 52 kDa.

Target

Images

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

  • All lanes : Anti-Sld2 antibody (ab139172) at 1 µg/ml

    Lane 1 : Exponentially Growing WT Yeast Whole Cell Lysate
    Lane 2 : W303 Yeast Lysate Whole Cell Lysate - Negative Control

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 52 kDa
    Observed band size: 61 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 29 kDa (possible non-specific binding), 42 kDa (possible non-specific binding), 67 kDa (possible non-specific binding)


    Exposure time: 30 seconds


    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab139172 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab139172 has not yet been referenced specifically in any publications.

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