Key features and details
- Rabbit polyclonal to SLD5
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-SLD5 antibody
See all SLD5 primary antibodies
DescriptionRabbit polyclonal to SLD5
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow
Recombinant fragment, corresponding to amino acids 1-198 of Human SLD5
- 293T, A431, Jurkat cell lines
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab101346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 26 kDa.|
|ICC/IF||1/100 - 1/500.|
|IHC-P||1/100 - 1/500. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).|
FunctionThe GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS4 is important for GINS complex assembly. GINS complex seems to bind preferentially to single-stranded DNA.
Sequence similaritiesBelongs to the GINS4/SLD5 family.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- DNA replication complex GINS protein SLD5 antibody
- GINS complex subunit 4 (Sld5 homolog) antibody
- GINS complex subunit 4 antibody
Anti-SLD5 antibody (ab101346) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 26 kDa
12% SDS PAGE
Anti-SLD5 antibody (ab101346) at 1/10000 dilution + Mouse heart at 50 µg
Predicted band size: 26 kDa
ab101346 at 1/500 staining SLD5 in paraffin-embedded human gastric cancer.
ab101346 at 1/200 staining paraformaldehyde-fixed A431 in IF. Bottom image costained with Hoechst 33342.
ab101346 has been referenced in 2 publications.
- Kaur M et al. Sld5 Ensures Centrosomal Resistance to Congression Forces by Preserving Centriolar Satellites. Mol Cell Biol 38:N/A (2018). PubMed: 29061732
- Kimura T et al. Induced expression of GINS complex is an essential step for reactivation of quiescent stem-like tumor cells within the peri-necrotic niche in human glioblastoma. J Cancer Res Clin Oncol N/A:N/A (2018). PubMed: 30465075