Overview

  • Product name

  • Description

    Rabbit polyclonal to SLD5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1-198 of Human SLD5

  • Positive control

    • 293T, A431, Jurkat cell lines

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab101346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
ICC/IF 1/100 - 1/500.
IHC-P 1/100 - 1/500. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).

Target

Images

  • Anti-SLD5 antibody (ab101346) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size: 26 kDa



    12% SDS PAGE
  • Anti-SLD5 antibody (ab101346) at 1/10000 dilution + Mouse heart at 50 µg

    Predicted band size: 26 kDa

  • ab101346 at 1/500 staining SLD5 in paraffin-embedded human gastric cancer.

  • ab101346 at 1/200 staining paraformaldehyde-fixed A431 in IF. Bottom image costained with Hoechst 33342.

References

This product has been referenced in:

  • Kaur M  et al. Sld5 Ensures Centrosomal Resistance to Congression Forces by Preserving Centriolar Satellites. Mol Cell Biol 38:N/A (2018). Read more (PubMed: 29061732) »
  • Kimura T  et al. Induced expression of GINS complex is an essential step for reactivation of quiescent stem-like tumor cells within the peri-necrotic niche in human glioblastoma. J Cancer Res Clin Oncol N/A:N/A (2018). Read more (PubMed: 30465075) »
See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab101346.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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