Overview

  • Product name
    Anti-SLD5 antibody
    See all SLD5 primary antibodies
  • Description
    Rabbit polyclonal to SLD5
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: VSIHQMEMER IRYVLSSYLR CRLMKIEKFF PHVLEKEKTR PEGEPSSLSP EELAFAREFM ANTESYLK, corresponding to amino acids 75-142 of Human SLD5. (Q9BRT9)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Human colon tissue; SLD5 over-expression HEK293T lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • Function
    The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS4 is important for GINS complex assembly. GINS complex seems to bind preferentially to single-stranded DNA.
  • Sequence similarities
    Belongs to the GINS4/SLD5 family.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
    • Alternative names
      • DNA replication complex GINS protein SLD5 antibody
      • GINS complex subunit 4 (Sld5 homolog) antibody
      • GINS complex subunit 4 antibody
      • GINS4 antibody
      • SLD5 antibody
      • SLD5 homolog antibody
      • SLD5 S cerevisiae homolog of antibody
      • SLD5_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLD5 antibody (ab121874)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLD5 antibody (ab121874)
      ab121874 at 1/500 dilution staining SLD5 in Paraffin-embedded Human Colon tissue by Immunohistochemistry.
    • Western blot - Anti-SLD5 antibody (ab121874)
      Western blot - Anti-SLD5 antibody (ab121874)
      All lanes : Anti-SLD5 antibody (ab121874) at 1/250 dilution

      Lane 1 : Negative control (vector only transfected HEK293T lysate)

      Lane 2 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells

    References

    ab121874 has not yet been referenced specifically in any publications.

    Publishing research using ab121874? Please let us know so that we can cite the reference in this datasheet.

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