Overview

  • Product name

    Anti-SLD5 antibody [EPR9318]
    See all SLD5 primary antibodies
  • Description

    Rabbit monoclonal [EPR9318] to SLD5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, IPmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SLD5 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • 293T cells; 293T, PC3, Jurkat and C6 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR9318
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab139683 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    Images

    • Flow cytometric analysis of permeabilized 293T cells labeling SLD5 with ab139683 at 1/100 dilution (red). Rabbit IgG negative control (green).
    • All lanes : Anti-SLD5 antibody [EPR9318] (ab139683) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : PC3 cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : C6 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 26 kDa

    References

    This product has been referenced in:

    • Köhler K  et al. The Cdk8/19-cyclin C transcription regulator functions in genome replication through metazoan Sld7. PLoS Biol 17:e2006767 (2019). Read more (PubMed: 30695077) »
    • Wu R  et al. H3K9me3 demethylase Kdm4d facilitates the formation of pre-initiative complex and regulates DNA replication. Nucleic Acids Res 45:169-180 (2017). WB . Read more (PubMed: 27679476) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab139683.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up