Key features and details
- Rabbit polyclonal to SLFN11
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SLFN11 antibody
DescriptionRabbit polyclonal to SLFN11
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
- Human pancreas tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121731 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 103 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Sequence similaritiesBelongs to the Schlafen family.
- Information by UniProt
- Schlafen family member 11 antibody
- SLFN11 antibody
- SLFN8/9 antibody
- SLN11_HUMAN antibody
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121731 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Anti-SLFN11 antibody (ab121731) + MOLT-4
Predicted band size: 103 kDa
Observed band size: 103 kDa
Western blot analysis in human cell line MOLT-4 staining SLFN11 with ab121731
ab121731 at 1/15 dilution staining SLFN11 in Paraffin-embedded Human Pancreas tissue by Immunohistochemistry. Note: moderate cytoplasmic positivity in islet cells.
ab121731 has not yet been referenced specifically in any publications.