Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-SLFN12 antibody [EPR20904-32] (ab234418)

Overview

  • Product name

    Anti-SLFN12 antibody [EPR20904-32]
    See all SLFN12 primary antibodies
  • Description

    Rabbit monoclonal [EPR20904-32] to SLFN12
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Fr, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SLFN12 aa 300-400. The exact sequence is proprietary.
    Database link: Q8IYM2

  • Positive control

    • WB: Wild-type HeLa whole cell lysate treated with DMSO or 17ß-estradiol. IHC-Fr: Human placenta tissue. Flow Cyt: HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20904-32
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab234418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 66 kDa.
IHC-Fr 1/100.
Flow Cyt 1/60.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    Images

    • Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen human placenta tissue labeling SLFN12 with ab234418 at 1/100 dilution (green), followed by Donkey anti-Rabbit IgG (H+L) secondary antibody, Alexa Fluor® 555 secondary at a 1/100 dilution. Positive staining in syncytiotrophoblast of human placenta is observed. Counterstained with DAPI (blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Donkey anti-Rabbit IgG (H+L) secondary antibody, Alexa Fluor® 555 used at a 1/100 dilution. Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).

      The image is kindly provided by Dr Xiaodong Wang.

    • All lanes : Anti-SLFN12 antibody [EPR20904-32] (ab234418) at 1/500 dilution

      Lane 1 : Wild-type HeLa (human cervix adenocarcinoma epithelial cell) treated with DMSO for 12 hours, whole cell lysate
      Lane 2 : Wild-type HeLa treated with 17ß-estradiol (5 µM) for 12 hours, whole cell lysate
      Lane 3 : SLFN12 knockout HeLa treated with DMSO for 12 hours, whole cell lysate
      Lane 4 : SLFN12 knockout HeLa treated with 17ß-estradiol (5 µM) for 12 hours, whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat at 1/2000 dilution

      Predicted band size: 66 kDa
      Observed band size: 67 kDa
      why is the actual band size different from the predicted?


      Exposure time: 5 seconds


      Blocking/Dilution buffer: 5% Defatted milk.

      The image is kindly provided by Dr Xiaodong Wang.

      ab234418 was shown to specifically react with SLFN12 in wild-type Hela cells as signal was lost in SLFN12 knockout cells. Wild-type and SLFN12 knockout samples were subjected to SDS-PAGE. ab234418 and anti-GAPDH antibody were incubated 8 hours at 4 °C at 1/500 dilution and 1/2000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (whole molecule), Peroxidase conjugated secondary antibody at 1/20,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

    • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cell line labeling SLFN12 with ab234418 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

    References

    This product has been referenced in:

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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