Overview

  • Product name

    Anti-SLFN5 antibody
  • Description

    Rabbit polyclonal to SLFN5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to internal sequence amino acids 70-210 of Human SLFN5.

  • Positive control

    • Human nasopharynx tissue; SK-MEL-30 cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nuclei but not nucleoli. Recommended concentration of ab121537 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121537 at 1/725 dilution staining SLFN5 in paraffin-embedded Human nasopharynx by Immunohistochemistry.
  • All lanes : Anti-SLFN5 antibody (ab121537) at 0.4 µg/ml

    Lane 1 : SK-MEL-30 cell lysate
    Lane 2 : Caco-2 cell lysate


    Loading control: Anti-HDAC1

References

This product has been referenced in:

  • Companioni Nápoles O  et al. SCHLAFEN 5 expression correlates with intestinal metaplasia that progresses to gastric cancer. J Gastroenterol 52:39-49 (2017). Read more (PubMed: 27032393) »
  • Ding L  et al. Schlafen 4-expressing myeloid-derived suppressor cells are induced during murine gastric metaplasia. J Clin Invest 126:2867-80 (2016). Read more (PubMed: 27427984) »
See all 2 Publications for this product

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