Overview

  • Product name

    Anti-Slingshot homolog 1/SSH1 antibody
    See all Slingshot homolog 1/SSH1 primary antibodies
  • Description

    Rabbit polyclonal to Slingshot homolog 1/SSH1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Mouse Slingshot homolog 1/SSH1 aa 450-550 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab123824)

  • Positive control

    • This antibody gave a positive signal in both Mouse Brain and Heart tissue lysates as well as A431 and HepG2 whole cell lysates.
  • General notes

     This product was previously labelled as Slingshot homolog 1

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab107799 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 115 kDa).

Target

  • Function

    Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein.
  • Sequence similarities

    Belongs to the protein-tyrosine phosphatase family.
    Contains 1 tyrosine-protein phosphatase domain.
  • Post-translational
    modifications

    Phosphorylated. Inhibitory phosphorylation by PAK4 promotes binding to YWHAZ. Phosphorylation at Ser-978 is decreased by stimuli which promote actin reorganization and lamellipodia formation. Can be dephosphorylated and activated by PPP3CA/calcineurin A. Phosphorylation decreases immediately prior to telophase.
  • Cellular localization

    Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cleavage furrow. Midbody. Also recruited to actin rich membrane protrusions such as lamellipodia, which may allow local control of actin dynamics at sites of cell locomotion. Also localized to the cleavage furrow and the midbody during cytokinesis.
  • Information by UniProt
  • Database links

  • Alternative names

    • AW551225 antibody
    • Coro1c antibody
    • coronin, actin binding protein 1C antibody
    • Gm1394 antibody
    • Gm1395 antibody
    • hSSH-1L antibody
    • mSSH 1L antibody
    • OTTMUSP00000031415 antibody
    • Protein phosphatase Slingshot homolog 1 antibody
    • Slingshot 1 antibody
    • slingshot 1L antibody
    • slingshot homolog 1 antibody
    • slingshot like 1 antibody
    • Slingshot1 antibody
    • SSH 1 antibody
    • SSH 1L antibody
    • SSH-1L antibody
    • SSH-like protein 1 antibody
    • Ssh1 antibody
    • SSH1_HUMAN antibody
    see all

Images

  • All lanes : Anti-Slingshot homolog 1/SSH1 antibody (ab107799) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Heart (Mouse) Tissue Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 115 kDa
    Observed band size: 150 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 56 kDa, 80 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes


    The predicted molecular weight of Slingshot homolog 1/SSH1 is 115 kDa (SwissProt), however we expect to observe a banding pattern around 150 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

References

ab107799 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (brain tissue)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
50 µg
Specification
brain tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 30 2016

Application
Western blot
Sample
Human Tissue lysate - whole (brain tissue)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
50 µg
Specification
brain tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 23 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain cortex)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
50 µg
Specification
Brain cortex
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 22 2015

Application
Western blot
Sample
Human Tissue lysate - whole (Brain cortex)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
50 µg
Specification
Brain cortex
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 22 2015

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