Product nameAnti-Slingshot homolog 1/SSH1 antibody
See all Slingshot homolog 1/SSH1 primary antibodies
DescriptionRabbit polyclonal to Slingshot homolog 1/SSH1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthetic peptide corresponding to Mouse Slingshot homolog 1/SSH1 aa 450-550 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in both Mouse Brain and Heart tissue lysates as well as A431 and HepG2 whole cell lysates.
This product was previously labelled as Slingshot homolog 1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab107799 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 115 kDa).|
FunctionProtein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family.
Contains 1 tyrosine-protein phosphatase domain.
modificationsPhosphorylated. Inhibitory phosphorylation by PAK4 promotes binding to YWHAZ. Phosphorylation at Ser-978 is decreased by stimuli which promote actin reorganization and lamellipodia formation. Can be dephosphorylated and activated by PPP3CA/calcineurin A. Phosphorylation decreases immediately prior to telophase.
Cellular localizationCytoplasm > cytoskeleton. Cell projection > lamellipodium. Cleavage furrow. Midbody. Also recruited to actin rich membrane protrusions such as lamellipodia, which may allow local control of actin dynamics at sites of cell locomotion. Also localized to the cleavage furrow and the midbody during cytokinesis.
- Information by UniProt
- AW551225 antibody
- Coro1c antibody
- coronin, actin binding protein 1C antibody
All lanes : Anti-Slingshot homolog 1/SSH1 antibody (ab107799) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Heart (Mouse) Tissue Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 115 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Additional bands at: 56 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
The predicted molecular weight of Slingshot homolog 1/SSH1 is 115 kDa (SwissProt), however we expect to observe a banding pattern around 150 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
ab107799 has not yet been referenced specifically in any publications.