Overview

  • Product name

    Anti-Slingshot homolog 1/SSH1 antibody
    See all Slingshot homolog 1/SSH1 primary antibodies
  • Description

    Rabbit polyclonal to Slingshot homolog 1/SSH1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human Slingshot homolog 1/SSH1 aa 675-725.
    Database link: NP_061857.2

  • Positive control

    • HeLa and 293T whole cell lysates.
  • General notes

     This product was previously labelled as Slingshot homolog 1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab76943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 116 kDa).
IP Use at 2-5 - 5 µg/mg of lysate.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function

    Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein.
  • Sequence similarities

    Belongs to the protein-tyrosine phosphatase family.
    Contains 1 tyrosine-protein phosphatase domain.
  • Post-translational
    modifications

    Phosphorylated. Inhibitory phosphorylation by PAK4 promotes binding to YWHAZ. Phosphorylation at Ser-978 is decreased by stimuli which promote actin reorganization and lamellipodia formation. Can be dephosphorylated and activated by PPP3CA/calcineurin A. Phosphorylation decreases immediately prior to telophase.
  • Cellular localization

    Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cleavage furrow. Midbody. Also recruited to actin rich membrane protrusions such as lamellipodia, which may allow local control of actin dynamics at sites of cell locomotion. Also localized to the cleavage furrow and the midbody during cytokinesis.
  • Information by UniProt
  • Database links

  • Alternative names

    • AW551225 antibody
    • Coro1c antibody
    • coronin, actin binding protein 1C antibody
    • Gm1394 antibody
    • Gm1395 antibody
    • hSSH-1L antibody
    • mSSH 1L antibody
    • OTTMUSP00000031415 antibody
    • Protein phosphatase Slingshot homolog 1 antibody
    • Slingshot 1 antibody
    • slingshot 1L antibody
    • slingshot homolog 1 antibody
    • slingshot like 1 antibody
    • Slingshot1 antibody
    • SSH 1 antibody
    • SSH 1L antibody
    • SSH-1L antibody
    • SSH-like protein 1 antibody
    • Ssh1 antibody
    • SSH1_HUMAN antibody
    see all

Images

  • All lanes : Anti-Slingshot homolog 1/SSH1 antibody (ab76943) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Predicted band size: 116 kDa
    Observed band size: 150 kDa
    why is the actual band size different from the predicted?

  • ab76943 at 1µg/ml staining Slingshot homolog 1/SSH1 in HeLa whole cell lysate immunoprecipitated using ab76943 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

  • ICC/IF image of ab76943 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76943, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Maimaiti Y  et al. SSH1 expression is associated with gastric cancer progression and predicts a poor prognosis. BMC Gastroenterol 18:12 (2018). Read more (PubMed: 29338701) »
  • Majumder P  et al. Cellular levels of Grb2 and cytoskeleton stability are correlated in a neurodegenerative scenario. Dis Model Mech 10:655-669 (2017). Read more (PubMed: 28360125) »
See all 9 Publications for this product

Customer reviews and Q&As

Answer

Thank you for contacting Abcam. Regarding the lysis/IP buffer to use with these antibodies, they have been tested with a buffer using the following formulation: IP lysis Buffer 12.5 ml 1M NaCl (250mM) 2.5 ml 1M Tris (50mM) 500ul 0.5M EDTA (5mM) 2.5ml 10% NP-40 32 ml dH20 That is not to say that RIPA will not work, however since these are cytoplasmic proteins, it would not be necessary. I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

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