Overview

  • Product name

  • Description

    Rabbit polyclonal to SLIRP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, WBmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse
  • Immunogen

    Synthetic peptide made to a C terminal region of human SLIRP.

  • Positive control

    • Human liver lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab51523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).

Target

  • Relevance

    SLIRP is a RNA binding protein that acts as a nuclear receptor corepressor. It may act by binding the SRA RNA, and repressing the SRA-mediated nuclear receptor coactivation. SLIRP binds the STR7 loop of SRA RNA. It is also able to repress glucocorticoid (GR), androgen (AR), thyroid (TR) and VDR-mediated transactivation. It contains 1 RRM (RNA recognition motif) domain. SLIRP is predominantly founs in mitochondrial with a limited role in the nucleus where it is recruited to nuclear receptor target promoters.
  • Cellular localization

    Mitochondrial and Nuclear; Predominantly mitochondrial.
  • Database links

  • Alternative names

    • C14orf156 antibody
    • Chromosome 14 open reading frame 156 antibody
    • DC50 antibody
    • PD04872 antibody
    • SLIRP antibody
    • SRA stem loop interacting RNA binding protein, mitochondrial antibody
    see all

Images

  • Anti-SLIRP antibody (ab51523) at 0.6 µg/ml + human liver lysate

    Predicted band size: 12 kDa
    Observed band size: 12 kDa

References

This product has been referenced in:

  • Carpp LN  et al. Quantitative proteomic analysis of host-virus interactions reveals a role for Golgi brefeldin A resistance factor 1 (GBF1) in dengue infection. Mol Cell Proteomics 13:2836-54 (2014). Read more (PubMed: 24855065) »
  • Clemente P  et al. hCOA3 stabilizes cytochrome c oxidase 1 (COX1) and promotes cytochrome c oxidase assembly in human mitochondria. J Biol Chem 288:8321-31 (2013). Read more (PubMed: 23362268) »
See all 6 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T, HCT116)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
HEK293T, HCT116
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Aug 31 2019

Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS cells)
Gel Running Conditions
Reduced Denaturing (4-12% Gradient Gel)
Loading amount
50 µg
Specification
U2OS cells
Blocking step
Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 03 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Normal skeletal muscle)
Specification
Normal skeletal muscle
Fixative
None
Permeabilization
No

Abcam user community

Verified customer

Submitted Mar 29 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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