Overview

  • Product name

  • Description

    Rabbit polyclonal to Slit1
  • Host species

    Rabbit
  • Specificity

    The smaller strong bands observed in the WB shown are likely to be cleavage products. A number of cleavage products for both Slit1 and Slit2 are reported in the literature resulting from alternate splicing and range from ~40kDa -160kDa, (Little et al, 2002).
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide:

    CSAKEQYFIPGTEDYHLN

    conjugated to KLH, corresponding to amino acids 487-504 of Mouse Slit1.

Properties

Applications

Our Abpromise guarantee covers the use of ab10984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 168 kDa (predicted molecular weight: 168 kDa).
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions (By similarity). SLIT1 and SLIT2 together seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb.
    • Tissue specificity

      Predominantly expressed in adult forebrain. Expressed in fetal brain, lung and kidney.
    • Sequence similarities

      Contains 1 CTCK (C-terminal cystine knot-like) domain.
      Contains 9 EGF-like domains.
      Contains 1 laminin G-like domain.
      Contains 20 LRR (leucine-rich) repeats.
      Contains 4 LRRCT domains.
      Contains 4 LRRNT domains.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • KIAA0813 antibody
      • MEGF 4 antibody
      • MEGF4 antibody
      • MGC164811 antibody
      • Multiple EGF-like domains protein 4 antibody
      • Multiple epidermal growth factor like domains 4 antibody
      • Multiple epidermal growth factor-like domains protein 4 antibody
      • OTTHUMP00000059392 antibody
      • SLIL 1 antibody
      • SLIL1 antibody
      • Slit 1 antibody
      • SLIT 3 antibody
      • Slit homolog 1 antibody
      • Slit homolog 1 protein antibody
      • Slit-1 antibody
      • Slit1 antibody
      • SLIT1_HUMAN antibody
      • SLIT3 antibody
      see all

    Images

    • ab10984-Rabbit polyclonal to Slit1 (1/500)

      Secondary ab: Goat anti-rabbit IgG ab6721 (1/5000); 20µg of cell lysate per lane. Expected molecular weight: 168 kDa; Exposure time: 5 min
           
      Lane 1: Rat Brain tissue lysate
      Lane 2: Mouse Brain tissue Lysate

      Note: The smaller strong bands observed in this WB are likely to be cleavage products. A number of  cleavage products for both Slit1 and Slit2 are reported in the literature resulting from alternate splicing and range from ~40kDa -160kDa, (Little et al, 2002).

      ab10984-Rabbit polyclonal to Slit1 (1/500) Secondary ab: Goat anti-rabbit HRP ab6721 (1/5000); 20µg of cell lysate per lane. Expected molecular weight: 168 kDa; Exposure time: 5 min Lane 1: Rat Brain tissue lysate Lane 2: Mouse Brain tissue Lysate Note: The smaller strong bands observed in this WB are likely to be cleavage products. A number of

    References

    ab10984 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    This product is known to not work in this application or species.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (e16 T/S whole embryo body)
    Specification
    e16 T/S whole embryo body
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid pH6
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C

    Mr. Carl Hobbs

    Verified customer

    Submitted Mar 22 2010

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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