Anti-Slit1 antibody [EP5796(N)] (ab183704)
Key features and details
- Rabbit monoclonal [EP5796(N)] to Slit1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
Anti-Slit1 antibody [EP5796(N)]
See all Slit1 primary antibodies -
Description
Rabbit monoclonal [EP5796(N)] to Slit1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal brain and forebrain lysates; SH-SY5Y cell lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP5796(N) -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183704 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/2000. Detects a band of approximately 200 kDa (predicted molecular weight: 168 kDa).
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Notes |
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WB
1/1000 - 1/2000. Detects a band of approximately 200 kDa (predicted molecular weight: 168 kDa). |
Target
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Function
Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions (By similarity). SLIT1 and SLIT2 together seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. -
Tissue specificity
Predominantly expressed in adult forebrain. Expressed in fetal brain, lung and kidney. -
Sequence similarities
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 9 EGF-like domains.
Contains 1 laminin G-like domain.
Contains 20 LRR (leucine-rich) repeats.
Contains 4 LRRCT domains.
Contains 4 LRRNT domains. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 6585 Human
- Omim: 603742 Human
- SwissProt: O75093 Human
- Unigene: 632082 Human
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Alternative names
- KIAA0813 antibody
- MEGF 4 antibody
- MEGF4 antibody
see all
Images
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Anti-Slit1 antibody [EP5796(N)] (ab183704) at 1/1000 dilution + SH-SY5Y cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 168 kDa -
All lanes : Anti-Slit1 antibody [EP5796(N)] (ab183704) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal forebrain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 168 kDa
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab183704 has not yet been referenced specifically in any publications.