Product nameAnti-Slit2 antibody [EPR2771]
See all Slit2 primary antibodies
DescriptionRabbit monoclonal [EPR2771] to Slit2
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Slit2 aa 1450-1550. The exact sequence is proprietary.
Database link: O94813
- A375 treated with TNF-a, A375, A549 and SH-SY5Y cell lysates, Human fetal kidney tissue
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab134166 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 169 kDa.|
|IP||1/10 - 1/100.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionThought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal chord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration.
Tissue specificityFetal lung and kidney, and adult spinal cord. Weak expression in adult adrenal gland, thyroid, trachea and other tissues examined.
Sequence similaritiesContains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 7 EGF-like domains.
Contains 1 laminin G-like domain.
Contains 20 LRR (leucine-rich) repeats.
Contains 4 LRRCT domains.
Contains 4 LRRNT domains.
DomainThe leucine-rich repeat domain is sufficient for guiding both axon projection and neuronal migration, in vitro.
Cellular localizationSecreted. The C-terminal cleavage protein is more diffusible than the larger N-terminal protein that is more tightly cell associated.
- Information by UniProt
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All lanes : Anti-Slit2 antibody [EPR2771] (ab134166) at 1/1000 dilution
Lane 1 : A375 treated with TNF-a cell lysates
Lane 2 : A375 cell lysates
Lane 3 : A549 cell lysates
Lane 4 : SH-SY5Y cell lysates
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 169 kDa
Immunohistochemical analysis of paraffin embedded Human fetal kidney tissue labelled with ab134166 at 1/100 dilution.
This product has been referenced in:
- Zhao SJ et al. SLIT2/ROBO1 axis contributes to the Warburg effect in osteosarcoma through activation of SRC/ERK/c-MYC/PFKFB2 pathway. Cell Death Dis 9:390 (2018). Read more (PubMed: 29523788) »
- Smeda M et al. Dual antiplatelet therapy with clopidogrel and aspirin increases mortality in 4T1 metastatic breast cancer-bearing mice by inducing vascular mimicry in primary tumour. Oncotarget 9:17810-17824 (2018). Read more (PubMed: 29707148) »