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RecombinantRabMAb

Recombinant Anti-Slit2 antibody [EPR2771] (ab134166)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (14)

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Western blot - Anti-Slit2 antibody [EPR2771] (ab134166)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit2 antibody [EPR2771] (ab134166)
  • Anti-Slit2 antibody [EPR2771] (ab134166)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2771] to Slit2
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-Slit2 antibody [EPR2771]
    See all Slit2 primary antibodies
  • Description

    Rabbit monoclonal [EPR2771] to Slit2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Slit2 aa 1450-1550. The exact sequence is proprietary.
    Database link: O94813

  • Positive control

    • WB: 293T whole cell lysate IHC: Human fetal kidney tissue
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR2771
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neural Signal Transduction
    • Neuroscience
    • Neurology process
    • Growth and Development
    • Axonal Guidance Proteins

Associated products

  • Alternative Versions

    • Anti-Slit2 antibody [EPR2771] - BSA and Azide free (ab240085)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • A549 whole cell lysate (ab7910)
  • Recombinant Protein

    • Recombinant human Slit2 protein (ab82131)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134166 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 169 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 169 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal chord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration.
    • Tissue specificity

      Fetal lung and kidney, and adult spinal cord. Weak expression in adult adrenal gland, thyroid, trachea and other tissues examined.
    • Sequence similarities

      Contains 1 CTCK (C-terminal cystine knot-like) domain.
      Contains 7 EGF-like domains.
      Contains 1 laminin G-like domain.
      Contains 20 LRR (leucine-rich) repeats.
      Contains 4 LRRCT domains.
      Contains 4 LRRNT domains.
    • Domain

      The leucine-rich repeat domain is sufficient for guiding both axon projection and neuronal migration, in vitro.
    • Cellular localization

      Secreted. The C-terminal cleavage protein is more diffusible than the larger N-terminal protein that is more tightly cell associated.
    • Target information above from: UniProt accession O94813 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 9353 Human
      • Entrez Gene: 20563 Mouse
      • Entrez Gene: 360272 Rat
      • Omim: 603746 Human
      • SwissProt: O94813 Human
      • SwissProt: Q9R1B9 Mouse
      • SwissProt: Q9WVC1 Rat
      • Unigene: 29802 Human
      • Unigene: 289739 Mouse
      • Unigene: 146652 Rat
      see all
    • Alternative names

      • Drad 1 antibody
      • E030015M03Rik antibody
      • E130320P19Rik antibody
      • FLJ14420 antibody
      • OTTHUMP00000158695 antibody
      • OTTHUMP00000217852 antibody
      • OTTHUMP00000217853 antibody
      • OTTHUMP00000217854 antibody
      • Slil 3 antibody
      • Slil3 antibody
      • Slit 2 antibody
      • Slit homolog 2 (Drosophila) antibody
      • Slit homolog 2 antibody
      • Slit homolog 2 protein antibody
      • Slit homolog 2 protein C-product antibody
      • Slit-2 antibody
      • Slit2 antibody
      • SLIT2_HUMAN antibody
      see all

    Images

    • Western blot - Anti-Slit2 antibody [EPR2771] (ab134166)
      Western blot - Anti-Slit2 antibody [EPR2771] (ab134166)
      Anti-Slit2 antibody [EPR2771] (ab134166) at 1/5000 dilution + 293T(Human embryonic kidney epithelial cell)whole cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 169 kDa
      Observed band size: 200,55 kDa why is the actual band size different from the predicted?



      Buffer and concentration: 5% NFDM /TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit2 antibody [EPR2771] (ab134166)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit2 antibody [EPR2771] (ab134166)

      Immunohistochemical analysis of paraffin embedded Human fetal kidney tissue labelled with ab134166 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-Slit2 antibody [EPR2771] (ab134166)
      Anti-Slit2 antibody [EPR2771] (ab134166)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (14)

    Publishing research using ab134166? Please let us know so that we can cite the reference in this datasheet.

    ab134166 has been referenced in 14 publications.

    • Zhan Y  et al. An MRI Study of Neurovascular Restorative After Combination Treatment With Xiaoshuan Enteric-Coated Capsule and Enriched Environment in Rats After Stroke. Front Neurosci 13:701 (2019). PubMed: 31354412
    • Lin YZ  et al. Roundabout signaling pathway involved in the pathogenesis of COPD by integrative bioinformatics analysis. Int J Chron Obstruct Pulmon Dis 14:2145-2162 (2019). PubMed: 31571851
    • Zeng Z  et al. Slit2-Robo2 signaling modulates the fibrogenic activity and migration of hepatic stellate cells. Life Sci 203:39-47 (2018). PubMed: 29660433
    • Fernando R  et al. Slit2 Modulates the Inflammatory Phenotype of Orbit-Infiltrating Fibrocytes in Graves' Disease. J Immunol 200:3942-3949 (2018). PubMed: 29752312
    • Zhao SJ  et al. SLIT2/ROBO1 axis contributes to the Warburg effect in osteosarcoma through activation of SRC/ERK/c-MYC/PFKFB2 pathway. Cell Death Dis 9:390 (2018). PubMed: 29523788
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    I bought Slit2 antibody (ab134166) for western blot. The datasheet is showing the predicted size is 169kDa. However there is huge band even in your datasheet with around 50kDa. Is there something wrong for the information? Please let me know about it.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 09 2013

    Answer

    We are assuming that the antibody is detecting the mature Slit homolog 2 protein C-product. The note that states that the predicted size is 169 kDa is referring to the precursor, before any processing of the protein by cleavage and other post-translation modifications. The UniProt database entry for the human protein lists some of these modifications:

    http://www.uniprot.org/uniprot/O94813 .

    Read More

    Tom Ruyle

    Abcam Scientific Support

    Answered on Jul 09 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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