Recombinant Anti-Slit2 antibody [EPR2771] (ab134166)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2771] to Slit2
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Slit2 antibody [EPR2771]
See all Slit2 primary antibodies -
Description
Rabbit monoclonal [EPR2771] to Slit2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Slit2 aa 1450-1550. The exact sequence is proprietary.
Database link: O94813 -
Positive control
- WB: 293T whole cell lysate IHC: Human fetal kidney tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2771 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134166 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 169 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 169 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal chord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration. -
Tissue specificity
Fetal lung and kidney, and adult spinal cord. Weak expression in adult adrenal gland, thyroid, trachea and other tissues examined. -
Sequence similarities
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 7 EGF-like domains.
Contains 1 laminin G-like domain.
Contains 20 LRR (leucine-rich) repeats.
Contains 4 LRRCT domains.
Contains 4 LRRNT domains. -
Domain
The leucine-rich repeat domain is sufficient for guiding both axon projection and neuronal migration, in vitro. -
Cellular localization
Secreted. The C-terminal cleavage protein is more diffusible than the larger N-terminal protein that is more tightly cell associated. - Information by UniProt
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Database links
- Entrez Gene: 9353 Human
- Entrez Gene: 20563 Mouse
- Entrez Gene: 360272 Rat
- Omim: 603746 Human
- SwissProt: O94813 Human
- SwissProt: Q9R1B9 Mouse
- SwissProt: Q9WVC1 Rat
- Unigene: 29802 Human
see all -
Alternative names
- Drad 1 antibody
- E030015M03Rik antibody
- E130320P19Rik antibody
see all
Images
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Anti-Slit2 antibody [EPR2771] (ab134166) at 1/5000 dilution + 293T(Human embryonic kidney epithelial cell)whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 169 kDa
Observed band size: 200,55 kDa why is the actual band size different from the predicted?Buffer and concentration: 5% NFDM /TBST
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit2 antibody [EPR2771] (ab134166)
Immunohistochemical analysis of paraffin embedded Human fetal kidney tissue labelled with ab134166 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab134166 has been referenced in 14 publications.
- Zhan Y et al. An MRI Study of Neurovascular Restorative After Combination Treatment With Xiaoshuan Enteric-Coated Capsule and Enriched Environment in Rats After Stroke. Front Neurosci 13:701 (2019). PubMed: 31354412
- Lin YZ et al. Roundabout signaling pathway involved in the pathogenesis of COPD by integrative bioinformatics analysis. Int J Chron Obstruct Pulmon Dis 14:2145-2162 (2019). PubMed: 31571851
- Zeng Z et al. Slit2-Robo2 signaling modulates the fibrogenic activity and migration of hepatic stellate cells. Life Sci 203:39-47 (2018). PubMed: 29660433
- Fernando R et al. Slit2 Modulates the Inflammatory Phenotype of Orbit-Infiltrating Fibrocytes in Graves' Disease. J Immunol 200:3942-3949 (2018). PubMed: 29752312
- Zhao SJ et al. SLIT2/ROBO1 axis contributes to the Warburg effect in osteosarcoma through activation of SRC/ERK/c-MYC/PFKFB2 pathway. Cell Death Dis 9:390 (2018). PubMed: 29523788