Overview

  • Product name

    Anti-Slit2 antibody [EPR2771]
    See all Slit2 primary antibodies
  • Description

    Rabbit monoclonal [EPR2771] to Slit2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Slit2 aa 1450-1550. The exact sequence is proprietary.
    Database link: O94813

  • Positive control

    • A375 treated with TNF-a, A375, A549 and SH-SY5Y cell lysates, Human fetal kidney tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134166 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 169 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal chord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration.
    • Tissue specificity

      Fetal lung and kidney, and adult spinal cord. Weak expression in adult adrenal gland, thyroid, trachea and other tissues examined.
    • Sequence similarities

      Contains 1 CTCK (C-terminal cystine knot-like) domain.
      Contains 7 EGF-like domains.
      Contains 1 laminin G-like domain.
      Contains 20 LRR (leucine-rich) repeats.
      Contains 4 LRRCT domains.
      Contains 4 LRRNT domains.
    • Domain

      The leucine-rich repeat domain is sufficient for guiding both axon projection and neuronal migration, in vitro.
    • Cellular localization

      Secreted. The C-terminal cleavage protein is more diffusible than the larger N-terminal protein that is more tightly cell associated.
    • Information by UniProt
    • Database links

    • Alternative names

      • Drad 1 antibody
      • E030015M03Rik antibody
      • E130320P19Rik antibody
      • FLJ14420 antibody
      • OTTHUMP00000158695 antibody
      • OTTHUMP00000217852 antibody
      • OTTHUMP00000217853 antibody
      • OTTHUMP00000217854 antibody
      • Slil 3 antibody
      • Slil3 antibody
      • Slit 2 antibody
      • Slit homolog 2 (Drosophila) antibody
      • Slit homolog 2 antibody
      • Slit homolog 2 protein antibody
      • Slit homolog 2 protein C-product antibody
      • Slit-2 antibody
      • Slit2 antibody
      • SLIT2_HUMAN antibody
      see all

    Images

    • All lanes : Anti-Slit2 antibody [EPR2771] (ab134166) at 1/1000 dilution

      Lane 1 : A375 treated with TNF-a cell lysates
      Lane 2 : A375 cell lysates
      Lane 3 : A549 cell lysates
      Lane 4 : SH-SY5Y cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 169 kDa

    • Immunohistochemical analysis of paraffin embedded Human fetal kidney tissue labelled with ab134166 at 1/100 dilution.

    References

    This product has been referenced in:

    • Zhao SJ  et al. SLIT2/ROBO1 axis contributes to the Warburg effect in osteosarcoma through activation of SRC/ERK/c-MYC/PFKFB2 pathway. Cell Death Dis 9:390 (2018). Read more (PubMed: 29523788) »
    • Smeda M  et al. Dual antiplatelet therapy with clopidogrel and aspirin increases mortality in 4T1 metastatic breast cancer-bearing mice by inducing vascular mimicry in primary tumour. Oncotarget 9:17810-17824 (2018). Read more (PubMed: 29707148) »
    See all 10 Publications for this product

    Customer reviews and Q&As

    Answer

    We are assuming that the antibody is detecting the mature Slit homolog 2 protein C-product. The note that states that the predicted size is 169 kDa is referring to the precursor, before any processing of the protein by cleavage and other post-translation modifications. The UniProt database entry for the human protein lists some of these modifications:

    http://www.uniprot.org/uniprot/O94813 .

    Read More

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