Product nameAnti-Slit2 antibody [SP122]
See all Slit2 primary antibodies
DescriptionRabbit monoclonal [SP122] to Slit2
Tested applicationsSuitable for: IHC-P, Flow Cytmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Pig
Synthetic peptide within Human Slit2 aa 1450 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: O94813
- IHC-P: Human prostate adenocarcinoma tissue. Flow Cyt: SH-SY5Y cells.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact firstname.lastname@example.org.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from TCS by protein A/G.
Our Abpromise guarantee covers the use of ab184856 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionThought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal chord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration.
Tissue specificityFetal lung and kidney, and adult spinal cord. Weak expression in adult adrenal gland, thyroid, trachea and other tissues examined.
Sequence similaritiesContains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 7 EGF-like domains.
Contains 1 laminin G-like domain.
Contains 20 LRR (leucine-rich) repeats.
Contains 4 LRRCT domains.
Contains 4 LRRNT domains.
DomainThe leucine-rich repeat domain is sufficient for guiding both axon projection and neuronal migration, in vitro.
Cellular localizationSecreted. The C-terminal cleavage protein is more diffusible than the larger N-terminal protein that is more tightly cell associated.
- Information by UniProt
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Flow Cytometry analysis of Neuro-2a (mouse neuroblastoma) cells labeling SLIT2 with purified ab184856 at 1:80 dilution (10.3 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab125938) / Black. Unlabeled control - Unlabelled cells / blue.
Flow cytometric analysis of rabbit anti-Slit2 (SP122) antibody ab184856 diluted 1/100 in SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells (green) compare to negative control of rabbit IgG (blue).
Staining of Slit2 in a Formalin/PFA-fixed paraffin-embedded section of human prostate adenocarcinoma tissue using ab184856 at a dilution of 1/100.
ab184856 has not yet been referenced specifically in any publications.